|
| Status |
Public on Mar 02, 2021 |
| Title |
Dynamic extrinsic pacing of the HOX clock in human axial progenitors control motor neuron subtype specification |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Rostro-caudal patterning of vertebrates depends on the temporally progressive activation of HOX genes within axial stem cells that fuel axial embryo elongation. Whether HOX genes sequential activation, the “HOX clock”, is paced by intrinsic chromatin-based timing mechanisms or by temporal changes in extrinsic cues remains unclear. Here, we studied HOX clock pacing in human pluripotent stem cells differentiating into spinal cord motor neuron subtypes which are progenies of axial progenitors. We show that the progressive activation of caudal HOX genes is controlled by a dynamic increase in FGF signaling. Blocking FGF pathway stalled induction of HOX genes, while precocious increase in FGF alone, or with GDF11 ligand, accelerated the HOX clock. Cells differentiated under accelerated HOX induction generated appropriate posterior motor neuron subtypes found along the human embryonic spinal cord. The HOX clock is thus dynamically paced by exposure parameters to secreted cues. Its manipulation by extrinsic factors alleviates temporal requirements to provide unprecedented synchronized access to human cells of multiple, defined, rostro-caudal identities for basic and translational applications.
|
| |
|
| Overall design |
human ES cells SA001
|
| |
|
| Contributor(s) |
Mouilleau V, Jarrige M, Martinat C, Nedelec S |
| Citation(s) |
33782043 |
| Submission date |
Jun 29, 2020 |
| Last update date |
Jul 01, 2021 |
| Contact name |
Stephane NEDELEC |
| E-mail(s) |
stephane.nedelec@inserm.fr
|
| Organization name |
Institut du Fer à Moulin
|
| Lab |
Stem cells and Neurodevelopment
|
| Street address |
17 rue du fer a moulin
|
| City |
PARIS |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA642885 |
| SRA |
SRP269264 |
Less...
More...