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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 23, 2021 |
| Title |
Expression data from malaria-specific mouse CD4 T cell (PbT-II) subset after Plasmodium chabaudi infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Upon activation, specific CD4+ T cells up-regulate the expression of CD11a and CD49d, surrogate markers of pathogen-specific CD4+ T cells. However, using T-cell receptor transgenic mice specific for a Plasmodium antigen, termed PbT-II, we found that activated CD4+ T cells develop not only to CD11ahiCD49dhi cells, but also to CD11ahiCD49dlo cells during acute Plasmodium infection. CD49dhi PbT-II cells, localized in the red pulp of spleens, expressed transcription factor T-bet and produced IFN-γ, indicating that they were type 1 helper T (Th1)-type cells. In contrast, CD49dlo PbT-II cells resided in the white pulp/marginal zones and were a heterogeneous population, with approximately half of them expressing CXCR5 and a third expressing Bcl-6, a master regulator of follicular helper T (Tfh) cells. In adoptive transfer experiments, both CD49dhi and CD49dlo PbT-II cells differentiated into CD49dhi Th1-type cells after stimulation with antigen-pulsed dendritic cells, while CD49dhi and CD49dlo phenotypes were generally maintained in mice infected with Plasmodium chabaudi. These results suggest that CD49d is expressed on Th1-type Plasmodium-specific CD4+ T cells, which are localized in the red pulp of the spleen, and can be used as a marker of antigen-specific Th1 CD4+ T cells, rather than that of all pathogen-specific CD4+ T cells.
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| Overall design |
PbT-II cells were adopted transferred into B6 mice and infected with Plasmodium chabaudi. Seven days after infection, we sorted subsets of PbT-II cells based on their expression of CD11a and CD49d expression.
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| Contributor(s) |
Jian J, Inoue S, Bayarsaikhan G, Miyakoda M, Kimura D, Kimura K, Nozaki E, Sakurai T, Fernandez-Ruiz D, Heath WR, Yui K |
| Citation(s) |
33914894, 34648636 |
| Submission date |
Jun 30, 2020 |
| Last update date |
Oct 21, 2021 |
| Contact name |
Katsuyuki Yui |
| E-mail(s) |
katsu@nagasaki-u.ac.jp
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| Organization name |
Nagasaki University
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| Lab |
Katsuyuki Yui
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| Street address |
7F, 1-12-4, Sakamoto
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| City |
Nagasaki |
| State/province |
Nagasaki |
| ZIP/Postal code |
8528523 |
| Country |
Japan |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (10)
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| GSM4647822 |
D7 Pcc CD11a_hi CD49d_hi PbT-II, biological rep1 |
| GSM4647823 |
D7 Pcc CD11a_hi CD49d_hi PbT-II, biological rep2 |
| GSM4647824 |
D7 Pcc CD11a_hi CD49d_hi PbT-II, biological rep3 |
| GSM4647825 |
D7 Pcc CD11a_hi CD49d_lo PbT-II, biological rep1 |
| GSM4647826 |
D7 Pcc CD11a_hi CD49d_lo PbT-II, biological rep2 |
| GSM4647827 |
D7 Pcc CD11a_hi CD49d_lo PbT-II, biological rep3 |
| GSM4647828 |
Navie PbT-II mouse CD11a_lo CD49d_lo, biological rep1 |
| GSM4647829 |
Navie PbT-II mouse CD11a_lo CD49d_lo, biological rep2 |
| GSM4647830 |
Navie PbT-II mouse CD11a_lo CD49d_lo, biological rep3 |
| GSM4647831 |
Navie PbT-II mouse CD11a_lo CD49d_lo, biological rep4 |
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| Relations |
| BioProject |
PRJNA643299 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE153600_RAW.tar |
10.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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