|
| Status |
Public on Jul 04, 2020 |
| Title |
Genome-Wide CRISPR Screens for Toxoplasma Gondii Genes Related to Artemisinin Susceptibility |
| Organisms |
Toxoplasma gondii; synthetic construct |
| Experiment type |
Other
|
| Summary |
We used CRISPR to generate pools of T. gondii mutants in the presence of dihydroartemisinin to identify genes that confer increased or decreased sensitivity to this drug.
|
| |
|
| Overall design |
We performed three screens in which we looked for mutations that decrease susceptibility to DHA and one screen in which we looked for mutations that increase susceptibility.
|
| |
|
| Contributor(s) |
Sidik S, Lourido S |
| Citation(s) |
32968076 |
| Submission date |
Jul 03, 2020 |
| Last update date |
Oct 03, 2020 |
| Contact name |
Sebastian Lourido |
| E-mail(s) |
lourido@wi.mit.edu
|
| Organization name |
Whitehead Institute
|
| Lab |
Lourido Lab
|
| Street address |
455 Main St.
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL15228 |
Illumina HiSeq 2000 (synthetic construct) |
| GPL16774 |
Illumina HiSeq 2000 (Toxoplasma gondii) |
|
| Samples (13)
|
|
| Relations |
| BioProject |
PRJNA644018 |
| SRA |
SRP269960 |
Less...
More...