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| Status |
Public on Jul 06, 2021 |
| Title |
Compartment-specific regulation of gene expression orchestrates homeostatic synaptic scaling |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Synaptic scaling is a form of homeostatic plasticity which allows neurons to reduce their action potential firing rate in response to chronic alterations in neural activity. Synaptic scaling requires profound changes in gene expression, but the relative contribution of local and cell-wide mechanisms to synaptic scaling is controversial. Here we performed a comprehensive multi-omics characterization of the somatic and process compartments of primary rat hippocampal neurons during synaptic scaling. Thereby, we uncovered highly compartment-specific and correlated changes in the neuronal transcriptome and proteome. Specifically, we identified highly compartment-specific downregulation of crucial regulators of neuronal excitability and excitatory synapse structure. Motif analysis further suggests an important role for trans-acting post-transcriptional regulators, including RNA-binding proteins and microRNAs, in the local regulation of the corresponding mRNAs. Altogether, our study indicates that compartmentalized gene expression changes are widespread in synaptic scaling and might co-exist with neuron-wide mechanism to allow synaptic computation and homeostasis.
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| Overall design |
Investigation of a compartment specific ptx effect on rat hippocampal neurons
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| Contributor(s) |
Schratt G, Colameo D, Rajman M, Soutschek M |
| Citation(s) |
34396684 |
| Submission date |
Aug 02, 2020 |
| Last update date |
Oct 05, 2021 |
| Contact name |
Gerhard Schratt |
| E-mail(s) |
gerhard.schratt@hest.ethz.ch
|
| Organization name |
ETH Zürich
|
| Department |
HEST
|
| Lab |
Lab of Systems Neuroscience
|
| Street address |
Winterthurerstrasse 190
|
| City |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA650210 |
| SRA |
SRP275504 |
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