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| Status |
Public on Sep 18, 2020 |
| Title |
Next Generation Sequencing of Chimeric antigen receptor T (CAR-T) cells polarized under Th9 condition |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Isolated human T cells was stimulated by anti CD3/CD28 Dynabeads and polarized under Th1 (IL2) or Th9 (IL4 plus TGF beta) condition.After 24 hours, cells were transduced with lentivirus. Medium were renewed every 2 days and cells were maintained at a concentration of 1 million per milliliter. On day 16 of culture total RNA was isolated from CAR-T cells using RNeasy Mini kits (Qiagen) following the manufacturer's instruction. Quality of total RNA was evaluated using RNA 6000 Nano LabChip. Qualified RNA was subject to next generation sequencing.
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| Overall design |
Next ggeneration sequencing was performed on chimeric antigen receptor T cells polarized under Th1 or Th9 condition.
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| Contributor(s) |
Liu L |
| Citation(s) |
33214555 |
| Submission date |
Aug 12, 2020 |
| Last update date |
Nov 30, 2020 |
| Contact name |
Lintao Liu |
| E-mail(s) |
liulintao@yahoo.com
|
| Organization name |
Houston Methodist Research Insititute
|
| Department |
Cancer
|
| Street address |
6550 FANNIN ST, RI-SMITH8-053
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA656703 |
| SRA |
SRP277150 |
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