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| Status |
Public on May 26, 2021 |
| Title |
Genome-scale CRISPR-Cas9 screen of Wnt/β-catenin signalling identifies therapeutic targets for Colorectal Cancer (RNA-seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To identify novel regulators and therapeutic targets of β-catenin-driven colorectal cancer development, genome-scale CRISPR-cas9 screens of Wnt/β-catenin were conducted and identified KMT2A as a key player of β-catenin-driven CRC progression. RNA-seq data was integrated with CHIP-seq and other experiments to assess the effect of KMT2A knockout on the transcriptomic profiling of CRC cells.
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| |
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| Overall design |
1) RNA samples from triplicates of NTC sgRNA-targeting DLD1 cells; 2) RNA samples from triplicates of two different KMT2A sgRNA-targeting DLD1 cells
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| |
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| Contributor(s) |
Firestein R, Mahara S, Wan C, Sun CX |
| Citation(s) |
34138730 |
|
https://doi.org/10.1126/sciadv.abf2567
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| Submission date |
Aug 12, 2020 |
| Last update date |
Jul 15, 2021 |
| Contact name |
Ron Firestein |
| E-mail(s) |
firesteinlab.ncbi@gmail.com
|
| Organization name |
Hudson Institute of Medical Research
|
| Department |
CCR
|
| Lab |
Functional Genomics
|
| Street address |
21 Wright street
|
| City |
Clayton |
| State/province |
VIC |
| ZIP/Postal code |
3168 |
| Country |
Australia |
| |
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| Platforms (1) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE156083 |
Genome-scale CRISPR-Cas9 screen of Wnt/β-catenin signalling identifies therapeutic targets for Colorectal Cancer |
|
| Relations |
| BioProject |
PRJNA656730 |
| SRA |
SRP277158 |
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