|
| Status |
Public on Nov 14, 2022 |
| Title |
Study of bone marrow Nestin+ mesenchymal stromal cells in control and leukemic mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
NRAS-G12D+ mice show reduced numbers of mesenchymal stromal cells together with increased apoptotic rates as compared to healthy controls.
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| Overall design |
Male Nes-gfp mice were lethally irradiated and transplanted with bone marrow cells from previously pI-pC induced control (Mx1-Cre-negative NRAS-G12D-positive) or leukemic (Mx1-Cre-positive NRAS-G12D-positive) mice, at the age of 28 weeks. Bone marrow Nes-gfp-positive mesenchymal stromal cells were FACS-sorted 4 weeks after the transplant, and total RNA was isolated and used for RNA sequencing.
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| Contributor(s) |
Bernal A, Utnes P, Paulssen RH, Arranz L |
| Citation(s) |
36596811 |
| Submission date |
Aug 28, 2020 |
| Last update date |
Feb 13, 2023 |
| Contact name |
Lorena Arranz |
| E-mail(s) |
lorena.arranz@uit.no
|
| Phone |
+47 77 62 08 41
|
| Organization name |
UiT The Arctic University of Norway
|
| Department |
Department of Medical Biology
|
| Lab |
Stem Cells, Ageing and Cancer Research Group
|
| Street address |
Hansine Hansens veg 74
|
| City |
Tromsø |
| ZIP/Postal code |
9019 |
| Country |
Norway |
| |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA659931 |
| SRA |
SRP279176 |
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