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| Status |
Public on Oct 23, 2009 |
| Title |
Effects of glucose transporter expression on VSMC |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Hypothesis: Overexpression of the GLUT1 facilitative glucose transporter, in A7r5 vascular smooth muscle cells, is sufficient and/or necessary to induce alterations in gene expression which influence apoptosis, growth, and proliferation.
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| Overall design |
Scientific Approach: A7r5 rat embryonic vascular smooth muscle cells (VSMCs) (ATCC, CRL-1444) were infected for 72 hours with adenoviral vectors containing human GLUT1 or empty vector controls at a multiplicity of infection of 10 (MOI of 1 = 100 particles/cell) and grown in both high glucose (25mM) and low glucose (5.5mM) media. The human GLUT1 cDNA adenoviral vector and empty vector controls were a gift from Dr Arno Kumagai (University of Michigan). Total RNA was isolated using TRIzol (Invitrogen Life Technologies, Carlsbad, CA), followed by extraction with phenol/chloroform until the interface was clear. Total RNA was further purified using Qiagen RNeasy Mini Kit (Qiagen 74106). Immunoblot analysis was conducted to confirm GLUT1 overexpression at the time of Total RNA isolation (72 hours post-infection) for each condition.
Number of chips: Total RNA was isolated from each of 2 conditions (control cells in low glucose (5.5mM), and GLUT1 overexpressing cells in low glucose), on three separate days and pooled to obtain one sample for each condition. In total we submitted two samples.
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| Contributor(s) |
Brosius FC, Vesely ED |
| Citation(s) |
19587217 |
| Submission date |
Apr 16, 2009 |
| Last update date |
Jul 26, 2019 |
| Contact name |
Eileen Danger Vesely |
| E-mail(s) |
evesely@umich.edu
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| Phone |
734-763-0980
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| Organization name |
University of Michigan
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| Department |
Nephrology
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| Lab |
Brosius, Frank C III
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| Street address |
1150 W. Medical Center Drive MSRB I. Room 5518
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL341 |
[RAE230A] Affymetrix Rat Expression 230A Array |
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| Samples (2) |
| GSM393216 |
Vascular Smooth muscle cells, control sample |
| GSM393277 |
Vascular Smooth muscle cells, GLUT1 over-expression sample |
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| Relations |
| BioProject |
PRJNA116605 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15713_RAW.tar |
5.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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