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| Status |
Public on Aug 31, 2020 |
| Title |
Expression of the olfactory receptors OR6X1 and OR4E2 decreases in hypothyroidism and is reversible with levothyroxine treatment in pregnancy |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
These libraries reflect a part of a large study. We construct transcriptome libraries, using whole blood circulating RNAs, of four healthy pregnancy (CTL) and preeclamptic women (PEC). In the manuscript, we perform an edgeR three-group analysis comparing the libraries of healthy thyroid pregnancy (HTP), gestational hypothyroidism (GHT) and preeclampsia (PEC). The HTP and GHT libraries are available in GSE147527. As a result, we obtained 1276 DEG in GHT and 1185 DEG in PEC. We also found 195 olfactory receptors common between only in GHT and PEC.
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| Overall design |
We constructed libraries of four healthy pregnancy (CTL) and four preeclamptic women (PEC). The whole blood was collected in the PAXgene Blood RNA tube (QIAGEN, NL, DE) and the RNA was extracted by PAXgene Blood RNA tube (QIAGEN, NL, DE). The sequencing was carried out using an Ion PI Chip (Thermo Fisher Scientific) in an Ion Proton Sequencer (Thermo Fisher Scientific).
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| Contributor(s) |
Hirata A, Almeida RJ, Rocha LA, Varela P, Bacigalupo LS, Curi FS, Rodrigues AG, Albejante MC, Silva LM, Silva VA, Pesquero JB, Delle H, Camacho CP |
| Citation(s) |
38069004 |
| Submission date |
Aug 30, 2020 |
| Last update date |
Dec 14, 2023 |
| Contact name |
Robson José de Almeida |
| Organization name |
Universidade Nove de Julho
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| Department |
Pós Graduação Stricto Sensu em Medicina
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| Lab |
Molecular Innovation and Biotechnology Laboratory
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| Street address |
RUA VERGUEIRO 235 249
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| City |
SAO PAULO |
| State/province |
SAO PAULO |
| ZIP/Postal code |
01504-001 |
| Country |
Brazil |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA660268 |
| SRA |
SRP279359 |
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