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| Status |
Public on Mar 23, 2021 |
| Title |
Ebp1-selective ribosome profiling analysis of Neuro2a cells with Ebp1-immuno-precipitation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We perform Selective Ribosome Profiling (SeRP) analysis of mouse Neuro2a neuronal cultures with Ebp1-immuno-precipitation in biological duplicate to assess the translation-specific function of Ebp1
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| Overall design |
Each biological replicate analyzed for ribosome-protected mRNA fragments after RNAse digestion in both Ebp-ribosome immuno-precipitation conditions, and the total input ribosome pool, followed by by high-throughput sequencing.
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| Contributor(s) |
Kraushar ML |
| Citation(s) |
33357414, 36482253 |
| Submission date |
Sep 02, 2020 |
| Last update date |
Jan 06, 2023 |
| Contact name |
Matthew Lee Kraushar |
| E-mail(s) |
matthew.kraushar@molgen.mpg.de
|
| Organization name |
Max Planck Institute for Molecular Genetics
|
| Department |
Department of Computational Molecular Biology
|
| Street address |
Ihnestrasse 63-73
|
| City |
Berlin |
| State/province |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
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| Platforms (1) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE157425 |
Protein synthesis in the developing neocortex at near-atomic resolution reveals Ebp1-mediated neuronal proteostasis at the 60S tunnel exit |
|
| Relations |
| BioProject |
PRJNA661060 |
| SRA |
SRP279771 |
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