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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 19, 2021 |
| Title |
Alarmin S100A8/A9 mediates activation of aberrant neutrophils in the pathogenesis of COVID-19 |
| Organisms |
Macaca mulatta; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To characterize the early immune responses against coronavirus infection, we infected rhesus macaques and hACE2 transgenic mice with SARS-CoV-2 and analyzed the transcriptome of infected and non-infected animals. We infected WT mice with IAV as a normal respiratory virus group. During analysis, we found that S100A8 was dramatically upregulated by SARS-CoV-2 and a mouse coronavirus (mouse hepatitis virus, MHV), but not by other tested viruses. A group of non-canonical neutrophils were also activated during SARS-CoV-2 infection.
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| Overall design |
WT Rhesus macaques were infected with SARS-CoV-2 intranasally. Lungs were harvested at 0 dpi, 3 dpi, and 5 dpi. RNA extracted from lungs and RNASeq was performed to identify differentially expressed genes.
WT C57BL/6J mice and hACE2 transgenic mice were infected with IAV and MHV intranasally. Lungs were harvested at 0 hour, 6 hour, 24 hour, 48hour, 3day, 5day, 7 day post-infection. RNA extracted from lungs and RNASeq was performed to identify differentially expressed genes.
Differentially expressed genes in Lungs of WT Rhesus macaques infected with SARS-CoV-2 and WT mice infected with IAV.
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| Contributor(s) |
Bao L, Guo Q, Guo X |
| Citation(s) |
33388094 |
| Submission date |
Sep 21, 2020 |
| Last update date |
Jan 19, 2021 |
| Contact name |
Xuefei Guo |
| E-mail(s) |
xuefeiguo@stu.pku.edu.cn
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| Organization name |
Peking university health science center
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| Department |
Institute of Systems Biomedicine
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| Lab |
You Lab
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| Street address |
Xueyuan road, haidian district
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| City |
Beijing |
| State/province |
Bejing |
| ZIP/Postal code |
100191 |
| Country |
China |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL14954 |
Illumina HiSeq 2000 (Macaca mulatta) |
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| Samples (44)
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| GSM4796678 |
Macaca mulatta lung with SARS-CoV2 at 0 dpi, 3 dpi, and 5 dpi. (A328) |
| GSM4796679 |
Macaca mulatta lung with SARS-CoV2 at 0 dpi, 3 dpi, and 5 dpi. (C205) |
| GSM4796680 |
Macaca mulatta lung with SARS-CoV2 at 0 dpi, 3 dpi, and 5 dpi. (D77) |
| GSM4796681 |
Macaca mulatta lung with SARS-CoV2 at 0 dpi, 3 dpi, and 5 dpi. (E202) |
| GSM4796682 |
Macaca mulatta lung with SARS-CoV2 at 0 dpi, 3 dpi, and 5 dpi. (F326) |
| GSM4796683 |
Mus musculus with MHV at 0 dpi, 1dpi, 3 dpi and 7 dpi. (EIL1) |
| GSM4796684 |
Mus musculus with MHV at 0 dpi, 1dpi, 3 dpi and 7 dpi. (WCL0) |
| GSM4796685 |
Mus musculus with MHV at 0 dpi, 1dpi, 3 dpi and 7 dpi. (WIL1) |
| GSM4796686 |
Mus musculus with MHV at 0 dpi, 1dpi, 3 dpi and 7 dpi. (WML1) |
| GSM4796687 |
Mus musculus with MHV at 0 dpi, 1dpi, 3 dpi and 7 dpi. (WML3) |
| GSM4796688 |
Mus musculus with MHV at 0 dpi, 1dpi, 3 dpi and 7 dpi. (WML7) |
| GSM4796689 |
hACE2 transgenic mice with SARS-CoV2 at 1dpi, 2dpi, 3dpi, 5dpi and 7dpi. (ACE2-1.BAM) |
| GSM4796690 |
hACE2 transgenic mice with SARS-CoV2 at 1dpi, 2dpi, 3dpi, 5dpi and 7dpi. (ACE2-20-1.BAM) |
| GSM4796691 |
hACE2 transgenic mice with SARS-CoV2 at 1dpi, 2dpi, 3dpi, 5dpi and 7dpi. (ACE2-20-2.BAM) |
| GSM4796692 |
hACE2 transgenic mice with SARS-CoV2 at 1dpi, 2dpi, 3dpi, 5dpi and 7dpi. (ACE2-3.BAM) |
| GSM4796693 |
hACE2 transgenic mice with SARS-CoV2 at 1dpi, 2dpi, 3dpi, 5dpi and 7dpi. (ACE2-5.BAM) |
| GSM4796694 |
hACE2 transgenic mice with SARS-CoV2 at 1dpi, 2dpi, 3dpi, 5dpi and 7dpi. (ACE2-7.BAM) |
| GSM4796695 |
Mus musculus with IAV and MHV at 6h, 24h, 48h. (IBDM-WT-CON) |
| GSM4796696 |
Mus musculus with IAV and MHV at 6h, 24h, 48h. (WT-IAV48-BMDM) |
| GSM4796697 |
Mus musculus with IAV and MHV at 6h, 24h, 48h. (WT-IV-24) |
| GSM4796698 |
Mus musculus with IAV and MHV at 6h, 24h, 48h. (WT-IV-6H) |
| GSM4796699 |
Mus musculus with IAV and MHV at 6h, 24h, 48h. (WT-MHV-6H) |
| GSM4796700 |
Mus musculus with IAV and MHV at 6h, 24h, 48h. (WTMHV-24-BMDM) |
| GSM4796701 |
Mus musculus with IAV and MHV at 6h, 24h, 48h. (WTMHV-48-BMDM) |
| GSM4796702 |
Macaca mulatta with SARS-CoV2 detected in different parts. (A.BAM) |
| GSM4796703 |
Macaca mulatta with SARS-CoV2 detected in different parts. (B.BAM) |
| GSM4796704 |
Macaca mulatta with SARS-CoV2 detected in different parts. (D.BAM) |
| GSM4796705 |
Macaca mulatta with SARS-CoV2 detected in different parts. (F.BAM) |
| GSM4796706 |
Macaca mulatta with SARS-CoV2 detected in different parts. (H.BAM) |
| GSM4796707 |
Macaca mulatta with SARS-CoV2 detected in different parts. (J.BAM) |
| GSM4796708 |
Macaca mulatta with SARS-CoV2 detected in different parts. (K.BAM) |
| GSM4796709 |
Macaca mulatta with SARS-CoV2 detected in different parts. (C.BAM) |
| GSM4796710 |
Macaca mulatta with SARS-CoV2 detected in different parts. (G.BAM) |
| GSM4796711 |
Macaca mulatta lung with SARS-CoV and SARS-CoV2. (Du-1.BAM) |
| GSM4796712 |
Macaca mulatta lung with SARS-CoV and SARS-CoV2. (Du-2.BAM) |
| GSM4796713 |
Mus musculus with IAV at 1dpi, 3dpi, 5dpi, 7dpi. (Day1.BAM) |
| GSM4796714 |
Mus musculus with IAV at 1dpi, 3dpi, 5dpi, 7dpi. (Day3.BAM) |
| GSM4796715 |
Mus musculus with IAV at 1dpi, 3dpi, 5dpi, 7dpi. (Day5.BAM) |
| GSM4796716 |
Mus musculus with IAV at 1dpi, 3dpi, 5dpi, 7dpi. (Day7.BAM) |
| GSM4796717 |
Mus musculus with IAV at 1dpi, 3dpi, 5dpi, 7dpi. (WT.BAM) |
| GSM4796718 |
Mus musculus with MHV at day 7dpi and rescue with medicine. (300.BAM) |
| GSM4796719 |
Mus musculus with MHV at day 7dpi and rescue with medicine. (301.BAM) |
| GSM4796720 |
Mus musculus with MHV at day 7dpi and rescue with medicine. (302.BAM) |
| GSM4796721 |
Mus musculus with MHV at day 7dpi and rescue with medicine. (303.BAM) |
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| Relations |
| BioProject |
PRJNA664767 |
| SRA |
SRP284264 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE158297_200427_A00262_0449_AH3CYVDSXY.xlsx |
3.3 Mb |
(ftp)(http) |
XLSX |
| GSE158297_200612_A00268_0484_BH5T7KDSXY.xlsx |
3.9 Mb |
(ftp)(http) |
XLSX |
| GSE158297_N2001400_YFP_80-429452914.xlsx |
4.6 Mb |
(ftp)(http) |
XLSX |
| GSE158297_N2001787_YFP_80-434261329.xlsx |
3.7 Mb |
(ftp)(http) |
XLSX |
| GSE158297_N2001794_YFP_80-434287925.xlsx |
4.2 Mb |
(ftp)(http) |
XLSX |
| GSE158297_N2001796_YFP_80-434295367.xlsx |
3.9 Mb |
(ftp)(http) |
XLSX |
| GSE158297_N2002260_YFP_80-440791514.xlsx |
10.8 Mb |
(ftp)(http) |
XLSX |
| GSE158297_N2003482_YFP_80-461806545.xlsx |
3.7 Mb |
(ftp)(http) |
XLSX |
| GSE158297_N2003508_GZR_80-461790558.xlsx |
3.5 Mb |
(ftp)(http) |
XLSX |
| GSE158297_N2004949_GZR_80-481563826.xlsx |
3.0 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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