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| Status |
Public on Jul 06, 2021 |
| Title |
The commensal bacterium Lactiplantibacillus plantarum imprints innate memory-like responses in mononuclear phagocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Gut microbiota is a constant source of antigens and stimuli to which the resident immune system has developed tolerance. However, the mechanisms by which mononuclear phagocytes, specifically monocytes/macrophages, cope with these usually pro-inflammatory signals is poorly understood. Here, we show that innate immune memory promotes anti-inflammatory homeostasis using as a model strains of the commensal bacterium, Lactiplantibacillus plantarum. Priming of monocytes/macrophages with bacteria, especially in its live form, enhances bacterial intracellular survival and decreases the release of pro-inflammatory signals to the environment, with lower production of TNF and higher levels of IL-10. Analysis of the transcriptomic landscape of these cells shows downregulation of pathways associated with the production of reactive oxygen species (ROS) and the release of cytokines, chemokines and antimicrobial peptides. Indeed, the induction of ROS prevents memory-induced bacterial survival. In addition, there is a dysregulation in gene expression of several metabolic pathways leading to decreased glycolytic and respiratory rates in memory cells. These data support commensal microbe-specific metabolic changes in innate immune memory cells that might contribute to homeostasis in the gut.
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| Overall design |
Genome-wide changes in gene expression in human monocytes exposed to culture medium, live or heat-killed L. plantarum
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| Contributor(s) |
Anguita J, Pellon A, Gonzalez M, Aransay AM |
| Citation(s) |
34224309 |
| Submission date |
Oct 13, 2020 |
| Last update date |
Jul 13, 2021 |
| Contact name |
Ana Maria Aransay |
| E-mail(s) |
amaransay@cicbiogune.es
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| Phone |
0034944061325
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| Organization name |
CIC bioGUNE
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| Department |
Genome Analysis Platform
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| Street address |
Parque tecnologico de Bizkaia, Building 801-A
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| City |
Derio |
| State/province |
BIZKAIA |
| ZIP/Postal code |
48160 |
| Country |
Spain |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (9)
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| GSM4831132 |
Monocytes primed with live L. plantarum Live1_6d |
| GSM4831133 |
Monocytes primed with live L. plantarum Live2_6d |
| GSM4831134 |
Monocytes primed with live L. plantarum Live3_6d |
| GSM4831135 |
Monocytes primed with heat-killed L. plantarum HK1_6d |
| GSM4831136 |
Monocytes primed with heat-killed L. plantarum HK2_6d |
| GSM4831137 |
Monocytes primed with heat-killed L. plantarum HK3_6d |
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| Relations |
| BioProject |
PRJNA669056 |
| SRA |
SRP287302 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE159496_JuAn-22_A_Raw_counts.tsv.gz |
357.5 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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