|
| Status |
Public on Feb 25, 2021 |
| Title |
In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions [Pofut1 ATAC-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The role of nutrient signaling processes in the fate decision of CD8 are incompletely understood. By performing in vivo pooled CRISPR-Cas9 screening, we uncovered nutrient signaling processes underpinning the dynamics and heterogeneity of CD8 T cell fate decisions.
|
| |
|
| Overall design |
We used ATAC seq to compare chromatin accessibility profiles of Pofut1 KO and Spike P14 cells from the dual-color transfer system at day 5 post-infection from spleen
|
| |
|
| Contributor(s) |
Huang H, Dhungana Y, Shi H, Chi H |
| Citation(s) |
33636132 |
| Submission date |
Oct 28, 2020 |
| Last update date |
Mar 01, 2021 |
| Contact name |
Hongbo Chi |
| E-mail(s) |
hongbo.chi@stjude.org
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Immunology
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (8)
|
|
| This SubSeries is part of SuperSeries: |
| GSE160341 |
In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions |
|
| Relations |
| BioProject |
PRJNA672934 |
| SRA |
SRP289484 |
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