 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 31, 2020 |
| Title |
Expression data from mouse heart tissue ischemia/reperfusion (I/R) treated for 0,24,48,72 hours |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Myocardial Ischemia-reperfusion injury is a serious clinical problem that lacks effective therapy.However, the precise mechanism leading to myocardial I/R injury remains unclear.Our study defined the dynamic changes of the differentially expressed genes related myocardial I/R injury, and provide basis for comprehensive understanding of the disease at molecular level.
We investigated schemia-reperfusion –mediated gene expression alteration associated with the development of cardiac injury by microarray in a mouse model.
|
| |
|
| Overall design |
All mice were euthanized by an overdose of pentobarbital on hours 6, 24 and 72 of ischemia/reperfusion (I/R) treated. Total RNA was isolated with TRIzol (Invitrogen) from heart organs (n=4 per group) at each time point according to manufacturer’s instructions. Gene expression profiling was performed using Affymetrix GeneChip mouse Genome 430 2.0 array according to the manufacturer’s instructions (Affymetrix, Inc., Santa Clara, CA). On the GeneChip Mouse Genome 430 2.0 Array, over 45,000 probe sets analyze the expression level of over 39,000 transcripts and variants from over 34,000 well characterized mouse genes.
|
| |
|
| Contributor(s) |
Zhang Y, Li H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 30, 2020 |
| Last update date |
Nov 02, 2020 |
| Contact name |
李 玉琳 |
| Organization name |
Beijing Institute of Heart Lung and Blood Vessel Diseases
|
| Street address |
2 Anzhen Rd, Chaoyang District,
|
| City |
Beijing |
| ZIP/Postal code |
100029 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA673424 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE160516_RAW.tar |
19.2 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...