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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 29, 2009 |
| Title |
Induction of Pluripotency in Adult Unipotent Germline Stem Cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Mouse and human stem cells with features similar to those of embryonic stem cells have been derived from testicular cells. Although pluripotent stem cells have been obtained from defined germline stem cells (GSCs) of mouse neonatal testis, only multipotent stem cells have been obtained so far from defined cells of mouse adult testis. In this study we describe a robust and reproducible protocol for obtaining germline-derived pluripotent stem (gPS) cells from adult unipotent GSCs. Pluripotency of gPS cells was confirmed by in vitro and in vivo differentiation, including germ cell contribution and transmission. As determined by clonal analyses gPS cells indeed originate from unipotent GSCs. We propose that the conversion process requires a GSC culture microenvironment that depends on the initial number of plated GSCs and the length of culture time.
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| Overall design |
Nine samples were analyzed.
GSC1: Mouse Germ Stem Cells, line 1 (1 replicate), GSC2: Mouse Germ Stem Cells, line 2 (1 replicate), GSC3: Mouse Germ Stem Cells, line 3 (1 replicate), GSC4: Mouse Germ Stem Cells, line 4 (1 replicate)
gPS1: Mouse clonal germ Pluripotent Stem cells, line 1, GFP-sorted (1 replicate), gPS2: Mouse clonal germ Pluripotent Stem cells, line 2, GFP-sorted (1 replicate), gPS3: Mouse clonal germ Pluripotent Stem cells, line 3, GFP-sorted (1 replicate)
ESC: Mouse Embryonic Stem Cells (duplicate)
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| Contributor(s) |
Ko K, Tapia N, Kim J, Araúzo-Bravo MJ, Sasse P, Glaser T, Wu G, Ruau D, Han D, Greber B, Hausdörfer K, Sebastiano V, Stehling M, Fleischmann BK, Brüstle O, Zenke M, Schöler HR |
| Citation(s) |
19570517 |
| Submission date |
May 20, 2009 |
| Last update date |
Jan 15, 2022 |
| Contact name |
Marcos J. Araúzo-Bravo |
| E-mail(s) |
mararabra@yahoo.co.uk
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| Phone |
+34 943 00 6108
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| Organization name |
Max Planck Institute for Molecular Biomedicine
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| Department |
Cell and Developmental Biology
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| Lab |
Computational Biology and Bionformatics
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| Street address |
Rogentstrasse
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| City |
Muenster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA115067 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16178_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR |
| GSE16178_non-normalized_data.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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