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| Status |
Public on Nov 20, 2020 |
| Title |
Gene expression analysis of normal primary human bronchial epithelial cells (pHBEC) stimulated by glucocorticoid, long-acting β2-adrenoceptor agonist or both combined. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In 2019, the Global Initiative for Asthma treatment guidelines were updated to recommend that inhaled corticosteroid (ICS)/long-acting β2-adrenoceptor agonist (LABA) combination therapy should be a first-in-line treatment option for asthma. Although clinically superior to ICS, mechanisms underlying the efficacy of this combination therapy remain unclear. We hypothesised the existence of transcriptomic interactions, an effect that was tested in primary human bronchial epithelial cells (pHBECs) using formoterol and budesonide as representative LABA and ICS, respectively.
To investigate the gene expression changes by each condition, transcriptomic analysis was performed on RNA extracted from pHBECs treated with budesonide, formoterol, or both combined.
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| Overall design |
Primary Human bronchial epithelial cells (pHBEC) were either not treated or treated with budesonide, formoterol or both combined for 6 h. Six independent experimental determinations, one from each different donor, were analyzed. Altogether, 24 arrays were hybridized.
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| Contributor(s) |
Newton R, Mostafa MM, Rider CF |
| Citation(s) |
33376135, 30704470 |
| Submission date |
Nov 19, 2020 |
| Last update date |
Feb 19, 2021 |
| Contact name |
Robert Newton |
| E-mail(s) |
rnewton@ucalgary.ca
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| Organization name |
University of Calgary
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| Department |
Snyder Institute for Chronic Diseases
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| Lab |
Airway Inflammation Research Group
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| Street address |
3330 Hospital Dr NW
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| City |
Calgary |
| State/province |
Alberta |
| ZIP/Postal code |
T2N 4N1 |
| Country |
Canada |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (24)
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| GSM4914824 |
pHBEC, control, 6h, donor 4 |
| GSM4914825 |
pHBEC, control, 6h, donor 5 |
| GSM4914826 |
pHBEC, control, 6h, donor 6 |
| GSM4914827 |
pHBEC, bud, 6h, donor 1 |
| GSM4914828 |
pHBEC, bud, 6h, donor 2 |
| GSM4914829 |
pHBEC, bud, 6h, donor 3 |
| GSM4914830 |
pHBEC, bud, 6h, donor 4 |
| GSM4914831 |
pHBEC, bud, 6h, donor 5 |
| GSM4914832 |
pHBEC, bud, 6h, donor 6 |
| GSM4914833 |
pHBEC, form, 6h, donor 1 |
| GSM4914834 |
pHBEC, form, 6h, donor 2 |
| GSM4914835 |
pHBEC, form, 6h, donor 3 |
| GSM4914836 |
pHBEC, form, 6h, donor 4 |
| GSM4914837 |
pHBEC, form, 6h, donor 5 |
| GSM4914838 |
pHBEC, form, 6h, donor 6 |
| GSM4914839 |
pHBEC, bud + form, 6h, donor 1 |
| GSM4914840 |
pHBEC, bud + form, 6h, donor 2 |
| GSM4914841 |
pHBEC, bud + form, 6h, donor 3 |
| GSM4914842 |
pHBEC, bud + form, 6h, donor 4 |
| GSM4914843 |
pHBEC, bud + form, 6h, donor 5 |
| GSM4914844 |
pHBEC, bud + form, 6h, donor 6 |
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| Relations |
| BioProject |
PRJNA679552 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE161805_RAW.tar |
51.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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