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| Status |
Public on Aug 05, 2021 |
| Title |
Identification of polysomal-association changes upon ablation of cytoplasmic SRSF1 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
SRSF1, shuttles between the nucleus and cytoplasm affecting post-splicing processes. However, the physiological significance of this remains unclear. Here, we used genome editing to knock-in a nuclear retention signal (NRS) in Srsf1 to create a mouse model harboring a non-shuttling SRSF1 protein. We then assessed how alblation of shuttling activities of SRSF1 affects mRNA translation.
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| Overall design |
Sequencing of total, monosomal, polysomal RNA from ESC, MEF and NSC cells expressing wild type or nuclear retained SRSF1
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| Contributor(s) |
Haward F, Maslon MM, Yeyati PL, Bellora N, Aitken S, von Kreigsheim A, Mill P, Adams IR, Cáceres JF |
| Citation(s) |
34338635 |
| Submission date |
Nov 19, 2020 |
| Last update date |
Aug 05, 2021 |
| Contact name |
Magdalena Maria Maslon |
| E-mail(s) |
magdalena.maslon@igmm.ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Street address |
Crewe Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH42XU |
| Country |
United Kingdom |
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| Platforms (1) |
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| Samples (50)
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| Relations |
| BioProject |
PRJNA679584 |
| SRA |
SRP293280 |
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