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| Status |
Public on Jan 20, 2022 |
| Title |
Transcriptional Inactivation of TP53 and BMP Pathway Components Mediates Therapy-induced Dedifferentiation and Metastasis in Prostate Cancer (RNA-seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
AR blockade instigates two separate and complementary processes: 1) transcriptional silencing of TP53 and hence acquisition of hybrid E/M and stem-like traits; and 2) inhibition of the BMP signaling, which promotes resistance to the pro-apoptotic and anti-proliferative effects of AR inhibition. The drug tolerant prostate cancer cells generated through reprogramming are rescued by neuregulin and generate metastases in mice.
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| Overall design |
Bulk RNA sequencing of the samples from various time points over the course of enzalutamide treatment of human prostate adenocarcinoma cells LNCaP; the samples from neuregulin rescued cells and LDN193189 treated cells, respectively. Also, we performed RNA-seq in the LNCaP cells with shRNA knock-down of TP53 or BRCA1.
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| Contributor(s) |
Wang Y, Han H, Rumandla A, Wang X, Giancotti F |
| Citation(s) |
35385726 |
| Submission date |
Nov 27, 2020 |
| Last update date |
Apr 21, 2022 |
| Contact name |
Filippo Giancotti |
| E-mail(s) |
fg2532@cumc.columbia.edu
|
| Organization name |
UT MD Anderson Cancer Center
|
| Department |
Cancer Biology
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| Lab |
Dr. Giancotti Lab
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| Street address |
1881 East Road
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77054 |
| Country |
USA |
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| Platforms (2) |
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| Samples (51)
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| This SubSeries is part of SuperSeries: |
| GSE162294 |
Transcriptional Inactivation of TP53 and BMP Pathway Components Mediates Therapy-induced Dedifferentiation and Metastasis in Prostate Cancer |
|
| Relations |
| BioProject |
PRJNA681148 |
| SRA |
SRP294453 |
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