 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 05, 2021 |
| Title |
Effect of the deletion of the lncRNA Neat1 on the rhythmic gene expression in the GH4C1 cell line |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study was to determine the effect of the deletion of the lncRNA Neat1 on the rhythmic gene expression in the GH4C1 cell line
|
| |
|
| Overall design |
GH4C1 sommatolactotroph pituitary cells (ATCC_ CCL-82.2™, Molsheim, France) were grown in HamF10 medium supplemented with 15% horse serum and 2% fetal calf serum. To synchronize cells between themselves, GH4C1 cells were transferred to fresh medium. Cells were harvested after this fresh medium replacement (T0) every 4 h from T10 to T34. At each time point, total RNA was prepared, and RNA was isolated using NucleoSpin®RNA XS (Macherey-Nagel).
|
| |
|
| Contributor(s) |
Jaq A, Becquet D, Guillen S, Boyer B, Goutierrez M, Fourel L, Franc J, François-Bellan A |
| Citation(s) |
33995907 |
|
https://doi.org/10.1016/j.csbj.2021.04.022
|
| Submission date |
Dec 07, 2020 |
| Last update date |
May 19, 2021 |
| Contact name |
Anne-Marie François-Bellan |
| E-mail(s) |
anne-marie.francois@univ-amu.fr
|
| Organization name |
CNRS-AMU
|
| Department |
Facute de Medecine
|
| Lab |
INP UMR 7051 CNRS-AMU
|
| Street address |
27 Bd Jean Moulin
|
| City |
Marseille |
| ZIP/Postal code |
13005 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
|
| Samples (42)
|
| GSM4959049 |
GH4C1 cells, WT at ZT14, rep1 |
| GSM4959050 |
GH4C1 cells, WT at ZT14, rep2 |
| GSM4959051 |
GH4C1 cells, WT at ZT14, rep3 |
| GSM4959052 |
GH4C1 cells, WT at ZT18, rep1 |
| GSM4959053 |
GH4C1 cells, WT at ZT18, rep2 |
| GSM4959054 |
GH4C1 cells, WT at ZT18, rep3 |
| GSM4959055 |
GH4C1 cells, WT at ZT22, rep1 |
| GSM4959056 |
GH4C1 cells, WT at ZT22, rep2 |
| GSM4959057 |
GH4C1 cells, WT at ZT22, rep3 |
| GSM4959058 |
GH4C1 cells, WT at ZT26, rep1 |
| GSM4959059 |
GH4C1 cells, WT at ZT26, rep2 |
| GSM4959060 |
GH4C1 cells, WT at ZT26, rep3 |
| GSM4959061 |
GH4C1 cells, WT at ZT30, rep1 |
| GSM4959062 |
GH4C1 cells, WT at ZT30, rep2 |
| GSM4959063 |
GH4C1 cells, WT at ZT30, rep3 |
| GSM4959064 |
GH4C1 cells, WT at ZT34, rep1 |
| GSM4959065 |
GH4C1 cells, WT at ZT34, rep2 |
| GSM4959066 |
GH4C1 cells, WT at ZT34, rep3 |
| GSM4959067 |
GH4C1 cells, KO at ZT10, rep1 |
| GSM4959068 |
GH4C1 cells, KO at ZT10, rep2 |
| GSM4959069 |
GH4C1 cells, KO at ZT10, rep3 |
| GSM4959070 |
GH4C1 cells, KO at ZT14, rep1 |
| GSM4959071 |
GH4C1 cells, KO at ZT14, rep2 |
| GSM4959072 |
GH4C1 cells, KO at ZT14, rep3 |
| GSM4959073 |
GH4C1 cells, KO at ZT18, rep1 |
| GSM4959074 |
GH4C1 cells, KO at ZT18, rep2 |
| GSM4959075 |
GH4C1 cells, KO at ZT18, rep3 |
| GSM4959076 |
GH4C1 cells, KO at ZT22, rep1 |
| GSM4959077 |
GH4C1 cells, KO at ZT22, rep2 |
| GSM4959078 |
GH4C1 cells, KO at ZT22, rep3 |
| GSM4959079 |
GH4C1 cells, KO at ZT26, rep1 |
| GSM4959080 |
GH4C1 cells, KO at ZT26, rep2 |
| GSM4959081 |
GH4C1 cells, KO at ZT26, rep3 |
| GSM4959082 |
GH4C1 cells, KO at ZT30, rep1 |
| GSM4959083 |
GH4C1 cells, KO at ZT30, rep2 |
| GSM4959084 |
GH4C1 cells, KO at ZT30, rep3 |
| GSM4959085 |
GH4C1 cells, KO at ZT34, rep1 |
| GSM4959086 |
GH4C1 cells, KO at ZT34, rep2 |
| GSM4959087 |
GH4C1 cells, KO at ZT34, rep3 |
|
| Relations |
| BioProject |
PRJNA682963 |
| SRA |
SRP296753 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE162751_ko_featurecounts.csv.gz |
606.8 Kb |
(ftp)(http) |
CSV |
| GSE162751_wt_featurecounts.csv.gz |
613.7 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...