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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 10, 2020 |
| Title |
microarray analysis of lung SPC/GFP+ EpCAM+ cells from different smoke exposure mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We extracted RNA of sorted lung SPC/GFP+ EpCAM+ cells and performed microarray analyses. Total RNA was extracted from sorted Ep-CAMhigh/GFPhigh cells using TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol. The RNA integrity was examined on an Agilent 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA). Biotinylated ss-cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA using the GeneChip WT PLUS Reagent Kit User Manual (Affymetrix/Thermo Fisher Scientific). Fragmented and labeled ss-cDNA were hybridized on a GeneChip Clariom S Array (Affymetrix/Thermo Fisher Scientific) (n =3/group).
We used microarrays to detail the global gene expression of SPC/GFP+ EpCAM+ cells from the control mice(G37-1_(Clariom_S_Mouse)), 3 week continuous smoke mice (G37-2_(Clariom_S_Mouse)) and 3 weeks intermittent smoke mice(G37-3_(Clariom_S_Mouse)), and identified distinct classes of up-regulated genes during this process.
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| Overall design |
Lung SPC/GFP+ EpCAM+ cells from the control mice, 3 week continuous smoke mice, and 3 week intermittent smoke mice were sorted, RNA extraction from these cells was hybridized on Affymetrix microarrays.
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| Contributor(s) |
Irie H |
| Citation(s) |
35236337 |
| Submission date |
Dec 09, 2020 |
| Last update date |
Mar 15, 2022 |
| Contact name |
Hidehiro Irie |
| E-mail(s) |
hidethecreek@gmail.com
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| Organization name |
Keio University
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| Street address |
35 Shinanomachi Shinjuku-ku
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| City |
Tokyo |
| ZIP/Postal code |
1608582 |
| Country |
Japan |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA683822 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE162919_RAW.tar |
3.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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