|
| Status |
Public on Dec 10, 2020 |
| Title |
O-GlcNAc regulates MTA1 transcriptional activity during breast cancer cells genotoxic adaptation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Chromatin modifier metastatic tumor protein 1 (MTA1), closely correlated with the development and progression in breast cancer, has a fantastic role in multiple cellular processes, including gene expression and cell homeostasis. Although MTA1 is a stress-responsive gene, its role in genotoxic adaptation remains unexplored. Here, we demonstrate that O-GlcNAc modification promotes MTA1 to interact with chromatin and regulates target gene expression, contributing to breast cancer cell genotoxic adaptation. MTA1 is modified with O-GlcNAc residues at serine 237/241/246 in adriamycin adaptive breast cancer cells and that modification improves the genome-wide interactions of MTA1 with gene promotor regions by enhancing its association with nucleosome remodeling and histone deacetylation (NuRD) complex. Further, O-GlcNAc-modulated MTA1 chromatin-binding influences the specific transcriptional regulation of genes involved in the adaptation of breast cancer cells to genotoxic stress.
We performed chromatin immunoprecipitation followed by sequencing (ChIP-seq) using anti-HA magnetic beads in HA-MTA1-WT or HA-MTA1-3A stably expressed MCF-7 cells. Next-generation sequencing libraries were generated and amplified for 15 cycles with BGISEQ kit. 100-300 bp DNA fragments were gel-purified and sequenced with BGISEQ-500 (BGI). Two biological replicates of the ChIP-seq were performed. We also analyzed gene expression in MTA1-WT and MTA1-3A expressed cells by RNA-seq.
|
| |
|
| Overall design |
MTA1 ChIP-seq was performed in HA-MTA1-WT and HA-MTA1-3A expressed MCF-7 cells using anti-HA magnetic beads. Gene expression profiling of HA-MTA1-WT and HA-MTA1-3A expressed MCF-7 cells were performed by RNA-seq.
|
| |
|
| Contributor(s) |
Xie X, Wu Q, Zhang K, Liu Y, Zhang N, Chen Q, Wang L, Li W, Zhang J, Liu Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 09, 2020 |
| Last update date |
Dec 12, 2020 |
| Contact name |
bo yu liu |
| E-mail(s) |
liuyubo@dlut.edu.cn
|
| Phone |
15842607600
|
| Organization name |
Dalian University of Technology
|
| Street address |
Dagong Road 2
|
| City |
panjin |
| State/province |
liaoning |
| ZIP/Postal code |
124000 |
| Country |
China |
| |
|
| Platforms (1) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA683874 |
| SRA |
SRP297403 |
Less...
More...