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| Status |
Public on Jan 03, 2023 |
| Title |
Elevated endogenous GDNF levels associate with schizophrenia in humans and mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Recent studies have identified presynaptic increase in striatal dopamine as the primary dopaminergic abnormality in schizophrenia, responsible for changes in cortical dopamine function in schizophrenic individuals. However, increased dopamine synthesis and release are not recapitulated in existing mouse models, limiting studies on disease progression and potential treatments. We found that the levels of glial cell line-derived neurotrophic factor (GDNF), a strong dopamine system modulator, are increased in the cerebrospinal fluid of first episode psychosis patients. Using a novel strategy to conditionally increase endogenous gene expression, we developed a mouse model with timed elevation of endogenous GDNF. Conditional ‘Knock Up’ of endogenous GDNF expression (GDNF cKU) in the central nervous system at midgestation resulted in an increase of dopamine levels in the presynaptic compartment of nigrostriatal dopamine neurons, hypodopaminergia in the prefrontal cortex (PFC) and schizophrenia-like behavioural deficits in the mouse. RNA sequencing from the PFC revealed gene expression changes similar to those previously found in schizophrenia patients, including downregulation of dopamine receptor 2 and adenosine A2a receptor (A2AR). Treatment of mice with A2AR antagonist istradefylline partially restored striatal and cortical dopamine levels, suggesting a potential therapeutic strategy to alleviate symptoms associated with schizophrenia. Taken together, our results demonstrate that timed increase in GDNF expression is sufficient to drive schizophrenia-like molecular and behavioural phenotypes, and represents a novel model for studying schizophrenia-associated pathologies in mice.
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| Overall design |
Transcriptomics analysis from mouse brain. Prefrontal cortex, striatum and substantia nigra were dissected from 2-month-old Nestin-Cre x GDNF cKU mice or from GDNF cKU mice 2 months after striatal AAV-Cre delivery.
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| Contributor(s) |
Mätlik K, Garton DR, Montaño-Rodríguez AR, Olfat S, Eren F, Casserly L, Damdimopoulos A, Panhelainen A, Porokuokka LL, Kopra JJ, Turconi G, Schweizer N, Bereczki E, Piehl F, Engberg G, Cervenka S, Piepponen TP, Zhang F, Sipilä P, Jakobsson J, Sellgren CM, Erhardt S, Andressoo J |
| Citation(s) |
35618883 |
| Submission date |
Dec 10, 2020 |
| Last update date |
Jan 03, 2023 |
| Contact name |
Anastasios Erik Damdimopoulos |
| Organization name |
Karolinska Institute
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| Department |
Department of Biosciences and Nutrition
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| Lab |
Bioinformatics and Expression Analysis Core Facility
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| Street address |
Hälsovägen 7c
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| City |
Stockholm |
| State/province |
Huddinge |
| ZIP/Postal code |
141 83 |
| Country |
Sweden |
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| Platforms (1) |
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| Samples (121)
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| Relations |
| BioProject |
PRJNA684069 |
| SRA |
SRP297549 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE162974_raw_counts.txt.gz |
5.6 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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