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| Status |
Public on Jun 07, 2021 |
| Title |
GATA6 defines endoderm fate by controlling chromatin accessibility during differentiation of human induced pluripotent stem cells [TF_ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Investigation of the role played by GATA6 in establishing the definitive endoderm chromatin accessbility profile. We used pluripotent stem cells as a model of early development. We derived GATA6-/- pluripotent cells with an inducible GATA6 or FOXA2 construct that permits exongenous GATA6 or FOXA2 cDNA expression upon supplementation of doxycycline. We differentiated GATA6+/+ and GATA6-/- (with and without doxycyline) cells to definitive endoderm and analyzed transcription factor binding profiles using CHIP-seq.
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| Overall design |
Examination of GATA6, EOMES and FOXA2 binding profiles during definitive endoderm formation in the absence and presence of GATA6.
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| Contributor(s) |
Heslop JA, Duncan SA |
| Citation(s) |
34010638 |
| Submission date |
Jan 04, 2021 |
| Last update date |
Jun 07, 2021 |
| Contact name |
Stephen A Duncan |
| E-mail(s) |
duncanst@musc.edu
|
| Phone |
843-792-9104
|
| Organization name |
Medical University South Carolina
|
| Department |
Regenerative Medicine and Cell Biology
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| Lab |
Duncan Lab
|
| Street address |
173 Ashley Avenue, BSB 6th Floor, Room 657A
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29425 |
| Country |
USA |
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| Platforms (1) |
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| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE156021 |
GATA6 defines endoderm fate by controlling chromatin accessibility during differentiation of human induced pluripotent stem cells. |
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| Relations |
| BioProject |
PRJNA689593 |
| SRA |
SRP300245 |
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