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Status |
Public on Jan 11, 2010 |
Title |
Heart Failure Associated Changes in Alternative Splicing of Sarcomere Genes |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Alternative mRNA splicing is an important mechanism for regulation of gene expression. Changes in gene expression contribute to the pathogenesis of heart failure. However, changes in mRNA splicing have not been systematically examined in heart disease. We hypothesized that mRNA splicing is changed in diseased hearts. We used the Affymetrix exon array, which separately measures expression of each known and computationally predicted exon, to globally evaluate changes in mRNA splicing in left ventricular myocardial RNA from control and ischemic cardiomyopathy (ICM) patients. We found that mRNA splicing is systematically changed in heart failure. RTPCR validated 9 previously unreported alternative splicing events. Furthermore, we demonstrated that the splicing of four key sarcomere genes, cardiac troponin T (TNNT2), cardiac troponin I (TNNI3), myosin heavy chain 7 (MYH7), and filamin C (FLNC), was significantly altered in ICM. Altered splicing of these genes in heart disease was confirmed in independent ICM samples, and in dilated cardiomyopathy and aortic stenosis. The minor variant fraction of these five genes was sufficient to classify samples into control or disease groups with 95% accuracy. Our data indicate that alternative splicing is systematically altered in human heart disease, suggesting that disease-associated perturbation of RNA splicing may contribute to the pathogenesis and development of heart failure. Disease-related changes in splicing of sarcomere genes may directly impact cardiomyocyte function.
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Overall design |
15 ischemic heart failure samples and 15 age and sex matched control heart samples were collected. Left ventricular myocardial samples, remote from infarcted areas, were obtained from explanted ischemic cardiomyopathy hearts at the time of transplantation, and from unused transplant donor hearts. Diseased hearts had ejection fraction < 40% and/or New York State Heart Association class III or class IV heart failure.
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Contributor(s) |
Kong SW, Pu WT |
Citation(s) |
20124440 |
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Submission date |
Jun 08, 2009 |
Last update date |
Feb 18, 2019 |
Contact name |
Sek Won Kong |
E-mail(s) |
swkong@enders.tch.harvard.edu
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Phone |
617-919-2689
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Organization name |
Boston Children's Hospital
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Department |
Informatics Program
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Lab |
EN137
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Street address |
300 Longwood Avenue
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
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Platforms (1) |
GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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Samples (30)
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GSM414642 |
Normal left ventricular function, Left Ventricle, sample 1 |
GSM414643 |
Normal left ventricular function, Left Ventricle, sample 2 |
GSM414644 |
Normal left ventricular function, Left Ventricle, sample 3 |
GSM414645 |
Normal left ventricular function, Left Ventricle, sample 4 |
GSM414646 |
Normal left ventricular function, Left Ventricle, sample 5 |
GSM414647 |
Normal left ventricular function, Left Ventricle, sample 6 |
GSM414648 |
Normal left ventricular function, Left Ventricle, sample 7 |
GSM414649 |
Normal left ventricular function, Left Ventricle, sample 8 |
GSM414650 |
Normal left ventricular function, Left Ventricle, sample 9 |
GSM414651 |
Normal left ventricular function, Left Ventricle, sample 10 |
GSM414652 |
Normal left ventricular function, Left Ventricle, sample 11 |
GSM414653 |
Normal left ventricular function, Left Ventricle, sample 12 |
GSM414654 |
Normal left ventricular function, Left Ventricle, sample 13 |
GSM414655 |
Normal left ventricular function, Left Ventricle, sample 14 |
GSM414656 |
Normal left ventricular function, Left Ventricle, sample 15 |
GSM414657 |
Ischemic heart failure, Left Ventricle, sample 1 |
GSM414658 |
Ischemic heart failure, Left Ventricle, sample 2 |
GSM414659 |
Ischemic heart failure, Left Ventricle, sample 3 |
GSM414660 |
Ischemic heart failure, Left Ventricle, sample 4 |
GSM414661 |
Ischemic heart failure, Left Ventricle, sample 5 |
GSM414662 |
Ischemic heart failure, Left Ventricle, sample 6 |
GSM414663 |
Ischemic heart failure, Left Ventricle, sample 7 |
GSM414664 |
Ischemic heart failure, Left Ventricle, sample 8 |
GSM414665 |
Ischemic heart failure, Left Ventricle, sample 9 |
GSM414666 |
Ischemic heart failure, Left Ventricle, sample 10 |
GSM414667 |
Ischemic heart failure, Left Ventricle, sample 11 |
GSM414668 |
Ischemic heart failure, Left Ventricle, sample 12 |
GSM414669 |
Ischemic heart failure, Left Ventricle, sample 13 |
GSM414670 |
Ischemic heart failure, Left Ventricle, sample 14 |
GSM414671 |
Ischemic heart failure, Left Ventricle, sample 15 |
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Relations |
BioProject |
PRJNA116119 |
Supplementary file |
Size |
Download |
File type/resource |
GSE16499_RAW.tar |
556.6 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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