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| Status |
Public on Mar 01, 2022 |
| Title |
Transcriptome analysis of JAK2V617F HSPC and endogenous HSPC from irradiated and non-irradiated legs of recipient mice transplanted with JAK2V617F hematopoietic cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
using transplantation of transgenic knock-in JAK2V617F hematopoietic cells into a single irradiated leg, we show development of Polycythemia Vera (PV) from a single anatomical site in immuno-competent mice. Mutant cells colonizing the non-irradiated leg efficiently induce PV. We compared transcriptomic profiles of sorted JAK2V617F HSPC and their reciprocal endogenous WT HSPC in the non-irradiated and irradiated legs from recipient mice transplanted with JAK2V617F hematopoietic cells.
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| Overall design |
we assesed the differentially expressed genes between endogenous WT and JAK2V617F sorted HSPC from the non-irradiated leg in order to find new potential therapeutic target for suppression of PV syndrome in this pre-clinical model through specific targeting of JAK2V617F cells.
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| Contributor(s) |
Lewandowski D, Murakami S |
| Citation(s) |
35290450 |
| Submission date |
Feb 01, 2021 |
| Last update date |
Jun 13, 2022 |
| Contact name |
Daniel LEWANDOWSKI |
| E-mail(s) |
daniel.lewandowski@cea.fr
|
| Phone |
+33 (0)1 46 54 86 04
|
| Organization name |
CEA
|
| Department |
IBFJ/iRCM
|
| Lab |
LRTS
|
| Street address |
18 route du Panorama
|
| City |
Fontenay aux Roses |
| State/province |
Ile-de-France |
| ZIP/Postal code |
92265 |
| Country |
France |
| |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA698571 |
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