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| Status |
Public on Jul 06, 2010 |
| Title |
Gene expression data from endothelial cells and leukocytes enriched from transplanted rat hearts |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptome analyses of organ transplants have until now usually focused on whole tissue samples containing activation profiles from different cell populations. Here, we enriched endothelial cells from rat cardiac allografts and isografts, establishing their activation profile at baseline and on day 2, 3 and 4 after transplantation. Modulated transcripts were assigned to three categories based on their regulation profile in allografts and isografts. Categories A and B contained the majority of transcripts and showed similar regulation in both graft types, appearing to represent responses to surgical trauma. By contrast, category C contained transcripts that were partly allograft-specific and to a large extent associated with interferon-gamma-responsiveness. Several transcripts were verified by immunohistochemical analysis of graft lesions, among them the matricellular protein periostin which was one of the most highly upregulated transcripts but has not been associated with transplantation previously. In conclusion, the majority of the differentially expressed genes in graft endothelial cells are affected by the transplantation procedure whereas relatively few are associated with allograft rejection.
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| Overall design |
Endothelial cells were enriched from control/non-transplanted hearts and from allografts (DA to Lewis) and isografts (Lewis to Lewis) harvested at day 2, 3 and 4 after transplantation. Three biological replicates from each time point and graft type were analyzed (a total of 24 arrays). Leukocytes were enriched from allografts at day 2 (n=1), day 3 (n=2) and day 4 (n=1) after transplantation.
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| Contributor(s) |
Mikalsen B, Fosby B, Wang J, Hammarström C, Bjærke H, Lundström M, Kasprzycka M, Scott H, Line P, Haraldsen G |
| Citation(s) |
20642680 |
| Submission date |
Jun 18, 2009 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Bjørg Mikalsen |
| Organization name |
Oslo University Hospital
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| Department |
Division of Pathology
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| Street address |
Sognsveien 20
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| City |
Oslo |
| ZIP/Postal code |
0027 |
| Country |
Norway |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA117379 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16695_RAW.tar |
75.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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