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| Status |
Public on Nov 01, 2009 |
| Title |
Expression data from adult rat tail MNs after spinal cord transection |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Spinal cord injury leads to impaired motor and sensory functions. After spinal cord injury there is a an initial phase of hypo-reflexia followed by a developing hyper-reflexia, often termed spasticity. Previous studies have suggested a relationship between the reappearence of plateau potentials in motor neurons and the development of spasticity after spinalization. To understand the molecular mechanism behind this phenomenon we examined the transcriptional response of the motor neurons after spinal cord injury.
We used a rat tail injury model where a complete transection of the caudal (S2) rat spinal cord leads to an immidate flaccid paralysis of the tail and a subsequent appearence of spasticity 2-3 weeks post injury that develops into strong spasticity after 2 months.
Gene expression changes were studied in motor neurons 21 and 60 days after complete spinal transection where the tail exhibits clear signs of spasticity.
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| Overall design |
Tail MNs were retorgradely labelled with flourogold injected into the muscle and intra peritoneally. 5-7 days after tracer injections the spinal cord was dissected out, snab frozen in liquid nitrogen, sliced in 10 um thick slices and fluorescent motor neurons were laser dissected into a collector tube to a total of ca. 50-200 cells pr sample. RNA was then extracted, two round amplified and hybridized to Affymetrix rat 230 2.0 arays. 27 samples were hybridized onto chips, 8 Spi-21, 8 Spi-60, 6 ShamC-21 and 5 ShamC-60.
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| Contributor(s) |
Ryge J, Wienecke J, Westerdahl A, Hultborn H, Kiehn O |
| Citation(s) |
19939961 |
| Submission date |
Jun 18, 2009 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Jesper Ryge |
| E-mail(s) |
jesper.ryge@epfl.ch
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| Phone |
+46707146879
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| Organization name |
karolinska institutet
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| Department |
neuroscience
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| Lab |
mammalian locomotor lab
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| Street address |
retziusvag 8
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| City |
stockholm |
| ZIP/Postal code |
17177 |
| Country |
Sweden |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (27)
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| GSM418660 |
Spi-21, biological rep 4 |
| GSM418661 |
Spi-21, biological rep 5 |
| GSM418662 |
Spi-21, biological rep 6 |
| GSM418663 |
Spi-21, biological rep 7 |
| GSM418664 |
Spi-21, biological rep 8 |
| GSM418665 |
Spi-60, biological rep 1 |
| GSM418666 |
Spi-60, biological rep 2 |
| GSM418667 |
Spi-60, biological rep 3 |
| GSM418668 |
Spi-60, biological rep 4 |
| GSM418669 |
Spi-60, biological rep 5 |
| GSM418670 |
Spi-60, biological rep 6 |
| GSM418671 |
Spi-60, biological rep 7 |
| GSM418672 |
Spi-60, biological rep 8 |
| GSM418673 |
ShamC-21, biological rep 1 |
| GSM418674 |
ShamC-21, biological rep 2 |
| GSM418675 |
ShamC-21, biological rep 3 |
| GSM418676 |
ShamC-21, biological rep 4 |
| GSM418677 |
ShamC-21, biological rep 5 |
| GSM418678 |
ShamC-21, biological rep 6 |
| GSM418679 |
ShamC-60, biological rep 1 |
| GSM418680 |
ShamC-60, biological rep 2 |
| GSM418681 |
ShamC-60, biological rep 3 |
| GSM418682 |
ShamC-60, biological rep 4 |
| GSM418683 |
ShamC-60, biological rep 5 |
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| Relations |
| BioProject |
PRJNA117361 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16710_RAW.tar |
64.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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