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| Status |
Public on Jul 06, 2021 |
| Title |
Suppression of plasmacytoid dendritic cell migration by compounds derived from natural medicines |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Background and Aims. Dendritic cells (DCs) play a pivotal role in maintaining immunological homeostasis by orchestrating innate and adaptive immune responses via migration to inflamed sites and the lymph nodes (LNs). Plasmacytoid DCs (pDCs) have been reported to accumulate in the colon of inflammatory bowel disease (IBD) patients and dextran sulfate sodium (DSS)-induced colitis mice. However, the role of pDCs in the progression of colonic inflammation remains unclear. Methods. 80 compounds in natural medicines were searched for inhibitors of pDC migration using bone marrow-derived pDCs (BMpDCs) and conventional DCs (BMcDCs). BALB/c mice were given 3% DSS in the drinking water for 7 days to induce acute colitis. Compounds, which specifically inhibited pDC migration, were administrated into DSS-induced colitis mice. Results. Astragaloside IV (As-IV) and oxymatrine (Oxy) suppressed BMpDC migration but not BMcDC migration. In DSS-induced colitis mice, the number of pDCs was markedly increased in the colonic lamina propria (LP), and the expression of CCL21 was obviously observed in colonic isolated lymphoid follicles (ILFs). As-IV and Oxy reduced symptoms of colitis and the accumulation of pDCs in colonic ILFs but not in the colonic LP. Moreover, in a BMpDC adoptive transfer model, BMpDC migration to colonic ILFs was significantly decreased by treatment with As-IV or Oxy. Conclusion. pDCs accumulated in the colon of DSS-induced colitis mice, and As-IV and Oxy ameliorated DSS-induced colitis by suppressing pDC migration to colonic ILFs. Accordingly, the selective inhibition of pDC migration may be a potential therapeutic approach for treating colonic inflammatory diseases.
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| Overall design |
To elucidate the mechanism underlying inhibitory effects of Astragaloside IV and Oxymatrine on pDC migration, BMpDCs were generated from bone marrow cells isolated from the femurs and tibias of male BALB/c mice. Mature BMpDCs were treated with Astragaloside IV or Oxymatrine for 12 hours, followed by induction with CCL21 for 1 hour at 37°C.
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| Contributor(s) |
Yamamoto T, Zhang Y, Hayashi S, Kadowaki M |
| Citation(s) |
34246191 |
| Submission date |
Feb 24, 2021 |
| Last update date |
Oct 11, 2021 |
| Contact name |
Takeshi Yamamoto |
| E-mail(s) |
ty@inm.u-toyama.ac.jp
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| Organization name |
University of Toyama
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| Street address |
2630 Sugitani
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| City |
Toyama |
| ZIP/Postal code |
930-0194 |
| Country |
Japan |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA704551 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE167388_RAW.tar |
3.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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