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Series GSE168506 Query DataSets for GSE168506
Status Public on Dec 01, 2022
Title Gene expression analysis in mouse mature T-cells either upon knockdown of L3MBTL3 or rescue with RBPJ WT or F261A/A284V mutant
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We identified genes upregulated upon depletion of L3MBTL3 in mouse mature T-cells (MT). Additionally, we identified genes downregulated upon rescue with RBPJ WT but not with an L3MBTL3-interacting defecting mutant (F261A/A284V) in MT cells depleted of RBPJ using the CRISPR/Cas9 technology.
 
Overall design RNA-seq analysis of mouse mature T-cells either upon knockdown of L3MBTL3 or rescue with RBPJ WT or F261A/A284V mutant.
 
Contributor(s) Friedrich T, Bartkuhn M, Giaimo BD, Ferrante F
Citation(s) 36477367
Submission date Mar 08, 2021
Last update date Jan 25, 2023
Contact name Tobias Friedrich
E-mail(s) tobias.friedrich@biochemie.med.uni-giessen.de
Organization name Justus-Liebig-University
Department Institute of Biochemistry
Street address Friedrichstr. 24
City Giessen
ZIP/Postal code 35392
Country Germany
 
Platforms (1)
GPL21626 NextSeq 550 (Mus musculus)
Samples (18)
GSM5144865 RNA-seq in mouse mature T-cells depleted of RBPJ and infected with an empty vector control, rep 1
GSM5144866 RNA-seq in mouse mature T-cells depleted of RBPJ and infected with a plasmid encoding RBPJ WT, rep 1
GSM5144867 RNA-seq in mouse mature T-cells depleted of RBPJ and infected with a plasmid encoding RBPJ F261A/A284V mutant, rep 1
Relations
BioProject PRJNA707592
SRA SRP309840

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE168506_normalized_counts_KD.csv.gz 374.6 Kb (ftp)(http) CSV
GSE168506_normalized_counts_rescue.csv.gz 657.3 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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