|
| Status |
Public on Mar 11, 2021 |
| Title |
miR-15a reprograms activated hepatic stellate cells back toward quiescence |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Cell reprogramming enables dedifferentiation to generate induced pluripotent stem cells or transdifferentiation to produce desired terminally differentiated cell types. However, the concept of forced reprogramming has not been extended to converting the activation status of specialized cell types such as hepatic stellate cell (HSC) which, once activated, plays a central role in promoting liver fibrosis. We identified miR-15a as a key driver to reprogram activated HSC back toward quiescence, partly by directly targeting Wisp1, to generate what we designate as induced quiescent-like HSC (iqHSC). iqHSCs had a morphologic, transcriptional, and functional phenotype similar to that of truly quiescent HSCs. Finally, performing cell therapy with iqHSCs attenuated hepatic inflammation and fibrosis in mouse models of liver injury induced by toxin, biliary obstruction, or diet.
|
| |
|
| Overall design |
We performed RNA sequencing of hepatic stellate cells in various activation states to predict a set of genes that have a higher likelihood of being targeted by miR-15a.
|
| |
|
| Contributor(s) |
Kim B, Zhu S, Hoshida Y, Choi MY |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 10, 2021 |
| Last update date |
Jun 29, 2022 |
| Contact name |
Shijia Zhu |
| E-mail(s) |
shijia.zhu@utsouthwestern.edu
|
| Organization name |
University of Texas Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
DALLAS |
| State/province |
Texas |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA713190 |
| SRA |
SRP310087 |
Less...
More...