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| Status |
Public on Mar 12, 2021 |
| Title |
Downregulation of TGR5 (GPBAR1) in biliary epithelial cells is characteristic of sclerosing cholangitis and may contribute to disease pathogenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Primary sclerosing cholangitis (PSC) is characterized by chronic inflammation and progressive fibrosis of the biliary tree. The bile acid receptor TGR5 is found on biliary epithelial cells (BECs), where it promotes secretion, proliferation and tight junction integrity. Thus, we speculated that changes in TGR5 expression in BECs may contribute to PSC pathogenesis.
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| Overall design |
PSC mouse models with genetic background Abcb4-/-, Abcb4-/-/Tgr5Tg and wt mice were generated (N=4 per group). Liver bile ducts enriched on BECs by FACS sorting with EpCAM were isolated. BEC gene expression was analysed by single cell transcriptomics (scRNAseq) from the different mouse models.
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| Contributor(s) |
Keitel V, Reich M, Koehrer K, Lautwein T, Vogel M, Mallm J, Gabernet G |
| Citation(s) |
33872692 |
| Submission date |
Mar 11, 2021 |
| Last update date |
Dec 08, 2022 |
| Contact name |
Gisela Gabernet |
| E-mail(s) |
gisela.gabernet@qbic.uni-tuebingen.de
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| Organization name |
Quantitative Biology Center (QBiC)
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| Department |
University of Tuebingen
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| Street address |
Auf der Morgenstelle 10
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| City |
Tuebingen |
| State/province |
BW |
| ZIP/Postal code |
72076 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA713826 |
| SRA |
SRP310380 |
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