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| Status |
Public on Mar 13, 2021 |
| Title |
Selenite induces cell cycle arrest and apoptosis through ROS-dependent inhibition of the AKT/mTOR pathway in thyroid cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We conducted a comprehensive RNA-seq analysis of early transcriptomic changes in KHM-5M to decipher the underlying mechanism of sodium selenite.
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| Overall design |
Thyroid cancer cells(KHM-5M) were treated with PBS (negative control) or sodium selenite respectively. Cells were harvested and Total RNA was extracted using Trizol reagent and purified by Oligo(dT)-attached magnetic beads. the single end 50 bases reads were generated on BGIseq500 platform (BGI-Shenzhen, China). The sequencing data was filtered with SOAPnuke, stored in FASTQ format, and mapped to HISAT2 (v2.0.4). The expression level was calculated by RSEM (v1.2.12).
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| Contributor(s) |
Cheng Z, Hong S, Xiao H |
| Citation(s) |
34094961 |
| Submission date |
Mar 12, 2021 |
| Last update date |
Jun 09, 2021 |
| Contact name |
Zhen Cheng |
| E-mail(s) |
chengzh25@mail2.sysu.edu.cn
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| Phone |
+8613602263913
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| Organization name |
The First Affiliated Hospital of Sun Yat-Sen University
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| Street address |
58 Zhongshan Er Road, Guangzhou
|
| City |
Guangzhou |
| State/province |
China |
| ZIP/Postal code |
510080 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA714134 |
| SRA |
SRP310463 |
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