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| Status |
Public on Jan 26, 2022 |
| Title |
Targeted CRISPR interference and activation of microRNA-3662-HBP1 axis controls tumor progression of triple-negative breast cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
MicroRNA-3662 (miR-3662) is not or very low expressed in normal human tissues but highly expressed in almost all types of human cancers, including breast cancer. However, the functional role of miR-3662 in human cancers remains a topic of debate. First, our bioinformatic analysis in the Cancer Genome Atlas dataset showed that miR-3662 expression is higher in triple-negative breast cancer (TNBC) and African American breast cancer than in other types of breast cancer. Next, we found that inhibition or knockout of endogenous mature miR-3662 in human TNBC cells suppresses cell proliferation and migration in vitro and tumor growth and metastasis in vivo. Functional analysis revealed that knockout of miR-3662 reduces the activation of Wnt/β-catenin signaling in TNBC cells. Finally, using dual-luciferase assay, miRNA/mRNA immunoprecipitation assay, and CRISPR-mediated miR-3662 activation and repression, we further identified that HMG-box transcription factor 1 (HBP-1), a Wnt/β-catenin signaling inhibitor, is a direct target of miR-3662 and is most likely responsible for miR-3662-mediated TNBC cell proliferation. Our results suggest that miR-3662 plays an oncogenic role in tumor progression and metastasis via a miR-3662-HBP1 axis, which negatively regulates Wnt /β-catenin signaling pathway in TNBC cells.
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| Overall design |
total RNA expression by deep-sequencing in MDA-MB-231 sorted for different levels of miR-3662 activity
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| Contributor(s) |
Wang L, Yi B, Liu R |
| Citation(s) |
34728806 |
| Submission date |
Mar 17, 2021 |
| Last update date |
Jan 26, 2022 |
| Contact name |
Lizhong Wang |
| E-mail(s) |
lwang12@uab.edu
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| Organization name |
UAB
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| Street address |
740 20th St S
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| City |
Birmingham |
| ZIP/Postal code |
35294 |
| Country |
USA |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA715248 |
| SRA |
SRP311153 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE169128_Processed_data_file.txt.gz |
522.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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