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| Status |
Public on Jun 26, 2010 |
| Title |
Expression profile of cell lines overexpressing miR-520g |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Primitive neuro-ectodermal tumours (PNET) of the supratentorial region are rare, highly malignant embryonal brain tumours affecting young children. We recently highlighted the importance of a microRNA cluster housing miR-520g. We utilized the Illumina HumanWG-6 v3 R2 and HumanHT-12 v3 R2 Expression BeadChip platforms to profile the effects of miR-520g on gene expression.
Keywords: gene expression, pediatric brain tumour, microRNA
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| Overall design |
We stably transfected human neural stem cells with miR-520g or pcDNA plasmid. They were cultured under differentiating conditions and subsequently harvested for microarray analysis. We performed two independent experiments with at least two technical replicates each.
Non-normalized data for Samples GSM425301-GSM425312 are available in the supplementary file 'GSE16988_GPL6884_non-normalized_data.txt'; non-normalized data for Samples GSM425313-GSM425318 are available in the supplementary file 'GSE16988_GPL6947_non-normalized_data.txt'.
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| Contributor(s) |
Li MH, Lee KF, Lu YT, Clarke I, Shih DJ, Picard D, Zhou L, Eberhart CG, Van Meter T, Lau C, Pomeroy S, Collins P, Modena P, Gajjar A, Taylor MD, Bouffet E, Rutka JT, Dirks PB, Hawkins CE, Huang A |
| Citation(s) |
19962671 |
| Submission date |
Jul 07, 2009 |
| Last update date |
Feb 18, 2019 |
| Contact name |
David J. H. Shih |
| E-mail(s) |
david.shih@utoronto.ca
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| Phone |
(416) 813-7654
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| Organization name |
Hospital for Sick Children
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| Department |
Developmental and Stem Cell Biology
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| Lab |
Peter Gilgan Centre for Research and Learning
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| Street address |
686 Bay St
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| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 0A4 |
| Country |
Canada |
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| Platforms (2) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA117335 |
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