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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 09, 2009 |
| Title |
Gene expression profiles of normal human mammary cell subpopulations FACS sorted based on expression of CD49f and EpCAM |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To delineate epithelial subpopulations in human mammary tissue, hematopoietic and endothelial cells were depleted from freshly isolated cell suspensions derived from reduction mammoplasties by fluorescence-activated cell sorting. The resultant Lin- population was fractionated into four distinct subpopulations using CD49f (α6-integrin) and epithelial cell adhesion molecule (EpCAM; also referred to as CD326 and ESA). Based on the immunohistochemical phenotype, and in vivo and in vitro functional assays, these subpopulations were identified as fibroblast-enriched stromal (CD49f -EpCAM-), mammary stem cell (MaSC)-enriched (CD49f hiEpCAM-), luminal progenitor (CD49f +EpCAM+), and mature luminal (CD49f –EpCAM+) cell subpopulations. Microarray profiling was used to derive gene expression signatures representative of these subpopulations using freshly sorted cells (>90% purity) from normal breast tissue. The four mammary cell subpopulations were found to have distinct gene expression profiles.
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| Overall design |
Four mammary cell subpopulations from three individual patient samples were analyzed.
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| Contributor(s) |
Smyth GK, Lindeman GJ, Visvader JE |
| Citation(s) |
19648928 |
| Submission date |
Jul 08, 2009 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Jane E Visvader |
| E-mail(s) |
visvader@wehi.edu.au
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| Phone |
(+61 3) 93452494
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| Fax |
(+61 3) 93470852
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| Organization name |
The Walter and Eliza Hall Institute of Medical Research
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| Department |
Victorian Breast Cancer Research Consortium
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| Lab |
Lindeman/Visvader Lab
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| Street address |
1G Royal Parade
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| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
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| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
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| Samples (12)
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| GSM425434 |
Patient 1, Lin-CD49fhiEpCAM- (MaSC-enriched) |
| GSM425435 |
Patient 1, Lin-CD49f-EpCAM- (Stromal) |
| GSM425436 |
Patient 1, Lin-CD49f-EpCAM+ (Mature luminal) |
| GSM425437 |
Patient 1, Lin-CD49f+EpCAM+ (Luminal progenitor) |
| GSM425438 |
Patient 2, Lin-CD49fhiEpCAM- (MaSC-enriched) |
| GSM425439 |
Patient 2, Lin-CD49f-EpCAM- (Stromal) |
| GSM425440 |
Patient 2, Lin-CD49f-EpCAM+ (Mature luminal) |
| GSM425441 |
Patient 2, Lin-CD49f+EpCAM+ (Luminal progenitor) |
| GSM425442 |
Patient 3, Lin-CD49fhiEpCAM- (MaSC-enriched) |
| GSM425443 |
Patient 3, Lin-CD49f-EpCAM- (Stromal) |
| GSM425444 |
Patient 3, Lin-CD49f-EpCAM+ (Mature luminal) |
| GSM425445 |
Patient 3, Lin-CD49f+EpCAM+ (Luminal progenitor) |
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| Relations |
| BioProject |
PRJNA117321 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16997_RAW.tar |
6.3 Mb |
(http)(custom) |
TAR |
| GSE16997_raw.txt.gz |
3.9 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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