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| Status |
Public on Jul 16, 2021 |
| Title |
A mechanism of coupling leading and lagging strand DNA synthesis under replication stress in budding yeast |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Other
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| Summary |
Here we show that the asymmetric DNA synthesis is also observed in mec1-100 and mrc1-AQ cells defective in replication checkpoint, but surprisingly, not in mrc1∆ cells in which both DNA replication and checkpoint functions of Mrc1 are missing. Furthermore, depletion of Mrc1 or its partner in DNA replication, Tof1, suppresses the asymmetric DNA synthesis in rad53-1 mutant cells.
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| Overall design |
ChIP-Seq was performed for Rfa1; BrdU was performed for yeast cells under different conditions.
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| Contributor(s) |
Serra-Cardona A, Yu C, Zhang X, Hua X, Gan H, Zhang Z |
| Citation(s) |
34531325 |
| Submission date |
Apr 14, 2021 |
| Last update date |
Jan 26, 2022 |
| Contact name |
Xu Hua |
| E-mail(s) |
xh2387@cumc.columbia.edu
|
| Organization name |
Columbia University
|
| Department |
Institute for Cancer Genetics
|
| Lab |
Zhiguo Zhang
|
| Street address |
1130 St Nicholas Ave
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA721981 |
| SRA |
SRP314853 |
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