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| Status |
Public on May 01, 2021 |
| Title |
Highly conserved sperm function-related transcripts across three species: human, rat and mouse. |
| Organisms |
Homo sapiens; Mus musculus; Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
mRNA sequences were analyzed in mouse, rat and human sperm samples to identify sperm transcriptomic similarities across species that could be used as biomarkers to predict male reproductive toxicity in animal models.
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| Overall design |
mRNA sequences were analyzed in mouse, rat and human sperm samples to identify sperm transcriptomic similarities across species that could be used as biomarkers to predict male reproductive toxicity in animal models. Semen specimens were collected from men aged 18 to 55 years with proven fertility. Rat and mouse semen specimens were collected via needle punctures of the cauda epididymides. Sperm RNAs were extracted using an optimized sperm RNA isolation protocol and subjected to polyA-purified mRNA-sequencing.
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| Contributor(s) |
Bianchi E, Boekelheide K, Hwang K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 30, 2021 |
| Last update date |
May 02, 2021 |
| Contact name |
Susan Hall |
| E-mail(s) |
susan_hall@brown.edu
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| Organization name |
Brown University
|
| Street address |
Box G-E5
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| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02912 |
| Country |
USA |
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| Platforms (3) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA726552 |
| SRA |
SRP318029 |
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