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| Status |
Public on May 11, 2021 |
| Title |
Transcriptome profile of S. cerevisiae strains expressing mistranslating tRNAs |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Mistranslation, the mis-incorporation of an amino acid not specified by the “standard” genetic code, occurs in all cells. tRNA variants that increase mistranslation arise spontaneously and engineered tRNAs can achieve mistranslation frequencies approaching 10% in yeast and bacteria. The goal of this study was to assess the transcriptome changes in yeast cells expressing two different mistranslating tRNA variants which mistranslate at similar frequencies (of ~ 3%) but result in different substitutions, namely alanine at proline codons or serine at arginine codons.
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| |
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| Overall design |
RNA sequencing of yeast strains expressing tRNA-Pro(G3:U70) or tRNA-Ser(UCU, G26A) compared to a wild-type yeast strain expressing no mistranslating tRNA
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| |
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| Contributor(s) |
Berg MD, Brandl CJ |
| Citation(s) |
34568909 |
| Submission date |
May 10, 2021 |
| Last update date |
Oct 06, 2021 |
| Contact name |
Matthew Berg |
| E-mail(s) |
mberg2@uwo.ca
|
| Organization name |
University of Western Ontario
|
| Department |
Biochemistry
|
| Street address |
1151 Richmond St
|
| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N6A5C1 |
| Country |
Canada |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA728585 |
| SRA |
SRP319155 |
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