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| Status |
Public on Aug 17, 2010 |
| Title |
eIF4E phosphorylation promotes tumorigenesis and is associated with prostate cancer progression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Translational regulation plays a critical role in cell growth and proliferation, and its dysregulation results in cancer. Aberrant expression of the mRNA 5’cap-binding protein, eIF4E, has been implicated in cancer development and progression. eIF4E activity is promoted by phosphorylation. Here we show that “knock-in” mice in which eIF4E cannot be phosphorylated are resistant to tumorigenesis in a prostate cancer model. We identify multiple candidate genes involved in the resistance to oncogenic transformation. Importantly, phosphorylation of eIF4E is increased in hormone-refractory prostate cancer, the deadliest stage of the disease. Our results highlight eIF4E phosphorylation as a critical event in tumorigenesis.
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| |
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| Overall design |
Comaparison of total RNA and polysomal RNA from mouse embryo fibroblasts derived from WT and eIF4E-KI (non phosphorylatable eIF4E) mice
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| |
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| Contributor(s) |
Larsson O, Sonenberg N |
| Citation(s) |
20679199 |
| Submission date |
Jul 31, 2009 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Ola Larsson |
| E-mail(s) |
ola.larsson@ki.se
|
| Phone |
+46 (0)8 517 73280
|
| Organization name |
Karolinska Institutet
|
| Department |
Department of oncology-pathology
|
| Street address |
CCK, R8:01
|
| City |
Stockholm |
| ZIP/Postal code |
171 76 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (8)
|
| GSM435171 |
Total RNA purified from MEFs derived from a EIF4E-KI mouse rep 1 |
| GSM435172 |
Polysomal RNA purified from MEFs derived from a EIF4E-KI mouse rep 1 |
| GSM435173 |
Total RNA purified from MEFs derived from a WT mouse rep 1 |
| GSM435174 |
Polysomal RNA purified from MEFs derived from a WT mouse rep 1 |
| GSM435175 |
Total RNA purified from MEFs derived from a WT mouse rep 2 |
| GSM435176 |
Polysomal RNA purified from MEFs derived from a WT mouse rep 2 |
| GSM435177 |
Polysomal RNA purified from MEFs derived from a EIF4E-KI mouse rep 2 |
| GSM435178 |
Total RNA purified from MEFs derived from a EIF4E-KI mouse rep 2 |
|
| Relations |
| BioProject |
PRJNA118901 |
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