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| Status |
Public on May 01, 2023 |
| Title |
Comprehensive analysis of the immune implication of NOTCH1 gene in stomach adenocarcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
In order to better understand the relationship between NOTCH1 gene and immunity in gastric adenocarcinoma cells, we performed the silent interference of NOTCH1 gene on three gastric adenocarcinoma cell lines AGS, MKN-45 and MGC-803. We extracted the total RNA from the interfered and untreated blank control cells, and performed lncRNA library construction and sequencing. The results of bioinformatics analysis showed that the silencing of NOTCH1 gene in gastric adenocarcinoma cells activated several immune-related signal transduction pathways, including T cell receptors, chemokines, JAK-STAT, Toll-like receptors, ECM receptors and other signals. Conduction pathway. These results confirm that NOTCH1 may down-regulate the immune response of gastric adenocarcinoma.
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| Overall design |
cell lines AGS, MKN-45 and MGC-803（ NOTCH1 interfered and untreated blank control） were used for RNA sequencing.
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| Contributor(s) |
Li L |
| Citation missing |
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| Submission date |
May 24, 2021 |
| Last update date |
May 01, 2023 |
| Contact name |
Liangqing Li |
| E-mail(s) |
liliangqing@fjmu.edu.cn
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| Organization name |
The First Affiliated Hospital of Fujian Medical University
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| Street address |
No 20, ChaZhong Road, TaiJiang District, Fuzhou 350000, China.
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| City |
Fuzhou |
| ZIP/Postal code |
050011 |
| Country |
China |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA732403 |
| SRA |
SRP321160 |
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