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Series GSE17676 Query DataSets for GSE17676
Status Public on Aug 14, 2010
Title Differential Effects of TiO2 Nanotubes on Vascular Cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The response of primary human endothelial (ECs) and vascular smooth muscle cells (VSMCs) to TiO2 nanotube arrays is studied through gene expression analysis. Microarrays revealed that nanotubes enhanced EC proliferation and motility, decreased VSMC proliferation, and decreased expression of molecules involved in inflammation and coagulation in both cell types. Network generated from significantly affected genes suggests that cells may be sensing nanotopographical cues via pathways previously implicated in sensing shear stress.
 
Overall design DNA microarrays were used to analyze the response of primary human endothelial (ECs) and vascular smooth muscle cells (VSMCs) to nanotopographical TiO2 surfaces. The experiment incorporated a 1 color design and used Agilent arrays that contained roughly 44,00 60mer probes that provide complete coverage of the human genome. 19 arrays were hybridized and represent 4 biological replicates for each group ( with the exception of group VSMC-NT30, which has 3 biological replicates). Data was analyzed separately for each cell type ( EC or VSMC). Gene expression comparisons were made between control cells grown on flat titanium (Ti) and cells grown on either 30nm nanotubes (NT30) or 100nm nantubes ( NT-100).
 
Contributor(s) Barczak A
Citation(s) 20030358
Submission date Aug 17, 2009
Last update date Jan 23, 2019
Contact name Andrea J Barczak
Organization name UCSF Sandler Center Functional Genomics Core Facility
Street address 1550 4th St Rock Hall 545
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
 
Platforms (1)
GPL6480 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)
Samples (19)
GSM441068 Hs primary aortic endothelial cells grown on flat titanium for 24 hrs_rep1
GSM441069 Hs primary aortic endothelial cells grown on flat titanium for 24 hrs_rep2
GSM441070 Hs primary aortic endothelial cells grown on flat titanium for 24 hrs_rep3
Relations
BioProject PRJNA118463

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17676_RAW.tar 103.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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