|
| Status |
Public on Mar 04, 2022 |
| Title |
RNA-sequencing of bovine fetal ovaries throughout gestation |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Understanding the developmental origins of reproductive disorders cannot be achieved without understanding normal development and the connected transcriptomics of the fetal ovary. To better understand this, we profiled and analysed RNA from fetal ovaries across gestation (62-276 days, n=19). The platform used detected 25,133 genes for each sample across gestation. Principal Component analysis of the data clustered the samples in three stages of development [early I (up to 98 days), early II (98-135 days) and late (> 135 days)]. Differential expression of genes between the three stages will then be used to delineate the pathways which are up-/down-regulated during these developmental stages.
|
| |
|
| Overall design |
Bovine fetal ovary mRNA profiles from two gestational stages, early (≤ 135 days, n=9) and late (> 136 days, n=10)
|
| |
|
| Contributor(s) |
Hummitzsch K, Azumah R, Rodgers RJ, Anderson RA, St.John J |
| Citation(s) |
35197999, 35413103 |
| Submission date |
Jun 18, 2021 |
| Last update date |
Jun 23, 2022 |
| Contact name |
Katja Hummitzsch |
| E-mail(s) |
katja.hummitzsch@adelaide.edu.au
|
| Organization name |
The University of Adelaide
|
| Department |
Obstetrics& Gynaecology
|
| Lab |
Katja Hummitzsch
|
| Street address |
Level 5, North Tce/George Street
|
| City |
Adelaide |
| State/province |
SA |
| ZIP/Postal code |
5005 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL23055 |
Illumina NextSeq 500 (Bos taurus) |
|
| Samples (19)
|
| GSM5391743 |
15/R12t: Fetal Ovary Early Gestation_rep1 |
| GSM5391744 |
15/R85t: Fetal Ovary Early Gestation_rep2 |
| GSM5391745 |
15/R86t: Fetal Ovary Early Gestation_rep3 |
| GSM5391746 |
15/R74t: Fetal Ovary Early Gestation_rep4 |
| GSM5391747 |
15/R41t: Fetal Ovary Early Gestation_rep5 |
| GSM5391748 |
15/R57t: Fetal Ovary Early Gestation_rep6 |
| GSM5391749 |
15/R42t: Fetal Ovary Early Gestation_rep7 |
| GSM5391750 |
15/R51t: Fetal Ovary Early Gestation_rep8 |
| GSM5391751 |
15/R43t: Fetal Ovary Early Gestation_rep9 [technical repeat - 2 raw files for this sample] |
| GSM5391752 |
15/R2t: Fetal Ovary Late Gestation_rep1 |
| GSM5391753 |
15/R44t: Fetal Ovary Late Gestation_rep2 |
| GSM5391754 |
15/R1t: Fetal Ovary Late Gestation_rep3 |
| GSM5391755 |
15/R45t: Fetal Ovary Late Gestation_rep4 |
| GSM5391756 |
15/R33t: Fetal Ovary Late Gestation_rep5 |
| GSM5391757 |
15/R47t: Fetal Ovary Late Gestation_rep6 |
| GSM5391758 |
15/R35t: Fetal Ovary Late Gestation_rep7 |
| GSM5391759 |
15/R38t: Fetal Ovary Late Gestation_rep8 |
| GSM5391760 |
15/R49t: Fetal Ovary Late Gestation_rep9 |
| GSM5391761 |
15/R50t: Fetal Ovary Late Gestation_rep10 |
|
| Relations |
| BioProject |
PRJNA739136 |
Less...
More...