|
| Status |
Public on Jun 23, 2021 |
| Title |
Yeast AlaRS mutants |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA sequencing of Saccharomyces cerevisiae wild type and alanyl-tRNA synthetase mutants grown at 30 degrees and challenged at 37 degrees
|
| |
|
| Overall design |
WT, AlaRS C719A and G906D mutant yeast strains were pregrown at 30 degrees, and shifted to 37 degrees for 1 or 2 hours. Cells before and after shifting to 37 degrees were collected and total RNA was prepared for Illumina sequencing
|
| |
|
| Contributor(s) |
Ling J, Zhang H |
| Citation(s) |
34500470 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R21 NS101245 |
Studies of Aminoacyl-tRNA Synthetase Mutations Causing Progressive Microcephaly |
UNIVERSITY OF MARYLAND |
JIQIANG LING |
| R35 GM136213 |
Regulation and Physiological Roles of Translational Fidelity |
UNIVERSITY OF MARYLAND |
JIQIANG LING |
|
| Submission date |
Jun 22, 2021 |
| Last update date |
Sep 22, 2021 |
| Contact name |
Jiqiang Ling |
| E-mail(s) |
jling12@umd.edu
|
| Phone |
3014051035
|
| Organization name |
The University of Maryland, College Park
|
| Department |
Cell Biology and Molecular Genetics
|
| Lab |
Ling Lab
|
| Street address |
2202 Microbiology Bldg., 4062 Campus Dr.
|
| City |
College Park |
| State/province |
MD |
| ZIP/Postal code |
20742 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
|
| Samples (27)
|
|
| Relations |
| BioProject |
PRJNA740022 |
| SRA |
SRP325110 |
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