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| Status |
Public on Dec 10, 2022 |
| Title |
G-quadruplex DNA as a target for pathogenic bacteria: efficacy of an extended naphthalene diimide ligand and mode of action |
| Organisms |
Escherichia coli; Staphylococcus aureus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Guanidine DNA quadruplex (G4-DNA) structures convey a distinctive layer of epigenetic information that is critical for the regulation of key biological activities and processes as genome transcription regulation, replication and repair. Despite several works that have been published recently, the information regarding their role and possible use as therapeutic drug targets in bacteria is still scarce. Here, we tested the biological activity of a small G4-DNA ligand library based on the naphthalene diimide (NDI) pharmacophore, against both Gram-positive and Gram-negative bacteria. For the best compound identified, NDI-10, the action mechanism was further characterized. Gram-negative bacteria were more resistant altogether due to the presence of the outer membrane, although the activity of the G4-Ligand was generally bactericidal, while it was bacteriostatic for Gram-positive bacteria. This asymmetric activity could be related to the different prevalence of putative G4-DNA structures in each group, the influence that they can exert on the gene expression (which was found more severe for the Gram-negative bacteria) and the role of the G4 structures in these bacteria, that seems to be more related to promote transcription in Gram-positive bacteria and repress transcription in Gram-negative.
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| Overall design |
4 samples Ecoli and 4 samples Saureus no replicates
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| Contributor(s) |
Cebrián R, Pirota V, Belmonte-Reche E, de Jong A, Morales JC, Freccero M, Doria F, Kuipers OP |
| Citation(s) |
34928608 |
| Submission date |
Jun 25, 2021 |
| Last update date |
Mar 13, 2023 |
| Contact name |
Anne de Jong |
| E-mail(s) |
anne.de.jong@rug.nl
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| Phone |
+31 50 363 2047
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| Organization name |
university of Groningen
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| Department |
Molecular Genetics
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| Street address |
Nijenborgh 7
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| City |
Groningen |
| ZIP/Postal code |
9747 AG |
| Country |
Netherlands |
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| Platforms (2) |
| GPL14548 |
Illumina HiSeq 2000 (Escherichia coli) |
| GPL17452 |
Illumina HiSeq 2000 (Staphylococcus aureus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA741414 |
| SRA |
SRP325617 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE178890_RAW.tar |
680.0 Kb |
(http)(custom) |
TAR (of RPKM) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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