Expression profiling by high throughput sequencing
Summary
The response to proteotoxic stresses such as heat shock is an ancient and ubiquitous transcriptional program allowing organisms to maintain protein homeostasis under changing environmental conditions. We depleted or deleted the three stress-specific transcription factors, Hsf1, Msn2 and Msn4, in S. cerevisiae and determined the effects on the transcriptome and proteome. Msn2/4 are responsible for a broad transcriptional reprogramming which includes i. a. the response to oxidative stress as well as biosynthesis of the protective sugar trehalose and glycolysis enzymes. Hsf1 activates the synthesis of molecular chaperones. In the absence of stress protection, thermal stress results in massive protein aggregation including many essential proteins. As a last resort to sustain life this triggers the transcriptomic induction of sporulation likely via the cell wall integrity signaling pathway.
Overall design
The dataset contains 20 different samples measured in triplicates (60 sequencing data sets): 4 strains at (25°C, 37°C HS after 10min and 30 min, 42°C HS after 10 Min and 30 min) = 4 strains x 5 conditions x 3 Replicates = 60 samples.
Less...
More...