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| Status |
Public on Jul 11, 2021 |
| Title |
Expression data from malaria-specific mouse CD4 T cell (PbT-II) subset after Plasmodium berghei ANKA infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The multiple species of Plasmodium that infect humans are highly polymorphic and induce various infections ranging from asymptomatic state to severe life-threatening diseases such as cerebral malaria. However, how the differences between the parasites affect host immune responses during blood-stage infection remains largely unknown. In this study, we investigated the CD4+ T-cell immune responses in T-cell receptor-transgenic mice infected simultaneously with P. berghei ANKA (PbA) and P. chabaudi chabaudi AS (Pcc) using PbT-II cells, which recognize a common epitope of these parasites. In the acute phase of infection, CD4+ T-cell responses in PbA-infected mice showed a higher involvement of Th1 cells and a lower proportion of Ly6Clo effector CD4+ T cells than those in Pcc-infected mice. Transcriptome analysis of PbT-II cells indicated that type I interferon (IFN)-regulated genes were expressed at higher levels in both Th1- and Tfh-type PbT-II cells from PbA-infected mice than those from Pcc-infected mice. Moreover, IFN-α levels were considerably higher in PbA-infected mice than in Pcc-infected mice. Inhibition of type I IFN signaling increased PbT-II and Ly6Clo cell numbers and partially reversed the Th1 over Tfh bias of the PbT-II cells in both PbA- and Pcc-infected mice. However, in the memory phase, we did not find a significant difference in the phenotype of PbT-II cells between PbA- and Pcc-primed mice. These observations suggested that the differences in Plasmodium-specific CD4+ T-cell responses between PbA- and Pcc-infected mice were at least partially associated with the difference in type I cytokine production during the early phase of the infection.
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| Overall design |
PbT-II cells underwent adoptive transfer into B6 mice and infected with Plasmodium berghei ANKA. Seven days after infection, we sorted subsets of PbT-II cells based on their expression of CD11a and CD49d expression.
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| Contributor(s) |
Ntita M, Inoue S, Jian J, Bayarsaikhan G, Kimura K, Kimura D, Miyakoda M, Nozaki E, Sakurai T, Fernandez-Ruiz D, Heath WR, Yui K |
| Citation(s) |
34648636 |
| Submission date |
Jul 10, 2021 |
| Last update date |
Oct 21, 2021 |
| Contact name |
Mbaya Ntita Felly |
| E-mail(s) |
mbayafeli@gmail.com
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| Phone |
+81 80-9107-8817
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| Organization name |
Nagasaki University
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| Department |
Molecular Microbiology and Immunology
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| Lab |
Immunology
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| Street address |
Sakamoto
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| City |
Nagasaki-shi |
| State/province |
Nagasaki |
| ZIP/Postal code |
852-8521 |
| Country |
Japan |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA745347 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE179860_RAW.tar |
6.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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