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| Status |
Public on Mar 31, 2011 |
| Title |
Dihydrotestosterone induced changes in gene expression in immortalized human conjunctival epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Genome-wide analysis of dihydrotestosterone (DHT) induced changes in gene expression in immortalized human conjunctival epithelial cells.
Analysis of regulation of immortalized human conjunctival epithelial cells by dihydrotestosterone at gene expression level. The hypothesis tested in the present study was that the androgen-eye interaction in ocular surface epithelial cells like conjunctival cells is influenced by androgens through regulation of the expression of multiple genes. Results provide important information of the differential regulation of numerous genes in response to dihydrotestosterone incubation of immortalized human conjunctival epithelial cells.
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| Overall design |
Total RNA was obtained from immortalized human conjunctival epithelial cells treated for 96 hours with 10 nM dihydrotestosterone (n=3) or vehicle (n=3). The RNA was then used with Illumina HumanHT-12 v3 Expression BeadChips to determine the effect of DHT on gene expression in an immortalized human conjunctival epithelial cell line developed in Dr. Rheinwald's laboratory [Rheinwald et al. MCB, 22 (14): 5157. (2002)] and charecterized in Dr. Ilene Gibson's laboratory [Gipson et al. IOVS, 44 (6): 2496. (2003)].
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| Contributor(s) |
Sullivan DA, Khandelwal P |
| Citation(s) |
22605918 |
| Submission date |
Sep 14, 2009 |
| Last update date |
Feb 18, 2020 |
| Contact name |
David A Sullivan |
| E-mail(s) |
david.sullivan@schepens.harvard.edu
|
| Phone |
(617) 912-0287
|
| Fax |
(617) 912-0101
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| Organization name |
Schepens Eye Research Institute
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| Lab |
David Sullivan
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| Street address |
20 Staniford Street
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (6)
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| GSM452311 |
Immortalized conjunctival epithelial cells Vehicle Treated Biological Replicate 1 |
| GSM452312 |
Immortalized conjunctival epithelial cells Vehicle Treated Biological Replicate 2 |
| GSM452313 |
Immortalized conjunctival epithelial cells Vehicle Treated Biological Replicate 3 |
| GSM452314 |
Immortalized conjunctival epithelial cells DHT Treated Biological Replicate 1 |
| GSM452315 |
Immortalized conjunctival epithelial cells DHT Treated Biological Replicate 2 |
| GSM452316 |
Immortalized conjunctival epithelial cells DHT Treated Biological Replicate 3 |
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| Relations |
| BioProject |
PRJNA119383 |
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