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| Status |
Public on Mar 01, 2022 |
| Title |
Expression data from E16 mouse embryonic organoids - grown with epithelium and stroma in the presence or absence of FGF2 [Epi] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
FGF2 signals BMP7 expression and TGFB/myofibroblast gene inhibition that promotes proacinar differentiation. We have characterized these stromal factors affecting the epithelium using a combination of scRNA Sequencing of whole tissue and transcriptional profiling of the organoid cell compartments.
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| Overall design |
Gene expression changes and GSEA was performed using gene expression microarrays. Organoids made from embryonic mouse cells were grown then different cell types were separated and their transcriptomes were analyzed based on their response to growth conditions with or without FGF2.
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| Contributor(s) |
Moskwa N, Mahmood A, Nelson DA, Larsen M |
| Citation(s) |
35224622 |
| Submission date |
Aug 03, 2021 |
| Last update date |
May 31, 2022 |
| Contact name |
Nicholas Moskwa |
| E-mail(s) |
nmoskwa@albany.edu
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| Organization name |
University at Albany
|
| Street address |
1400 Washington Avenue
|
| City |
Albany |
| State/province |
New York |
| ZIP/Postal code |
12222 |
| Country |
USA |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE181430 |
Single-cell sequencing reveals PDFGRα+ stromal cell subpopulations that promote proacinar differentiation in embryonic salivary gland organoids |
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| Relations |
| BioProject |
PRJNA751931 |
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