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| Status |
Public on May 20, 2022 |
| Title |
Epigenomic and transcriptomic features induced by silencing of ZNF280C in colon cancer cells and knock-out of Zfp280c in mice [ChIP-Seq, ATAC-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Znic finger proteins play crucial roles in development and disease, including tumorigenesis. Here, we identifed ZNF280C as a novel oncogenic driver in colorectal cancer, and systematically studied the molecular mechanisms underlying ZNF280C-mediated malignant transformation and progression.
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| Overall design |
RKO colon cancer cells stably expressing a ZNF280C-targeting shRNA controlled by a tetracycline-inducible promoter were treated with or without 1 ug/ml doxycycline for 72 hours, and the cells were harvested for analysis as described below. For transgenic mice experiments, Zfp280c knock-out or wild-type C57BL/6J mice were sacrificed at 12 weeks of age and colon tissues were havested for transriptomic analysis as described below.
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| Contributor(s) |
Shu X |
| Citation(s) |
35605119 |
| Submission date |
Aug 11, 2021 |
| Last update date |
Aug 24, 2022 |
| Contact name |
Xingsheng Shu |
| E-mail(s) |
shuxingsheng@gmail.com
|
| Organization name |
Shenzhen University
|
| Department |
Health Science Center
|
| Street address |
No 3688, Nanhai Avenue
|
| City |
Shenzhen |
| State/province |
Guangdong |
| ZIP/Postal code |
518060 |
| Country |
China |
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| Platforms (2) |
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| Samples (29)
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| This SubSeries is part of SuperSeries: |
| GSE181856 |
Zinc finger protein 280C contributes to colorectal tumorigenesis by maintaining epigenetic repression at H3K27me3-marked loci |
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| Relations |
| BioProject |
PRJNA753710 |
| SRA |
SRP332045 |
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