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| Status |
Public on Dec 31, 2021 |
| Title |
Novel functional analysis of MET exon 14 skipping alteration in driving cancer invasion and metastatic dissemination |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
MET exon 14 skipping alteration (MET∆14Ex) is an actionable oncogenic driver that occurs in 2-4% of non-small cell lung cancer (NSCLC) cases. However, the precise role of MET∆14Ex in tumor progression in NSCLC is poorly understood. We here demonstrate that MET∆14Ex expression increases receptor kinase activity and downstream signaling by impairing receptor internalization and endocytic degradation, which significantly boosts tumor cell scattering and invasion as well as metastasis. To explore the key mechanisms in regulation of these phenotypes, RNA sequencing (RNA-seq) studies were conducted on HGF-treated MET∆14Ex and MET cell models in an effort to identify the global change of transcriptome signature and key functional pathways involved in MET∆14Ex-medieated tumorigenesis. Both Ingenuity pathway analysis (IPA) and gene set enrichment analysis (GSEA) revealed an induction of a tight cluster of functional pathways associated with cell movement in HGF-treated MET∆14Ex models (vs MET) in a time-dependent manner.
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| Overall design |
In order to gain insights into the key molecular pathways involved in the biological effects of METD14Ex, an RNA-seq study was conducted on MET and METD14Ex cells treated with HGF for 0, 6 and 24h. Then, both Ingenuity pathway analysis (IPA) and gene set enrichment analysis (GSEA) were conducted to identify the global change of transcriptome signature and key functional pathways involved in MET∆14Ex-medieated tumorigenesis.
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| Contributor(s) |
Feng W, Yang L, Haiying C, Balazs H |
| Citation missing |
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| Submission date |
Aug 24, 2021 |
| Last update date |
Jan 02, 2022 |
| Contact name |
Feng Wang |
| E-mail(s) |
feng.wang.202108@gmail.com
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| Organization name |
albert einstein college of medicine
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| Street address |
1300 Morris Park Ave
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| City |
Bronx |
| ZIP/Postal code |
10461 |
| Country |
USA |
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| Platforms (1) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA757418 |
| SRA |
SRP333918 |
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